化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2013年
7期
987-993
,共7页
细胞色素c%2-巯基嘧啶%循环伏安法%化学修饰电极%直接电化学
細胞色素c%2-巰基嘧啶%循環伏安法%化學脩飾電極%直接電化學
세포색소c%2-구기밀정%순배복안법%화학수식전겁%직접전화학
cytochrome c%2-mercaptopyrimidine%cyclic voltammetry%chemically modified electrode%direct electrochemistry
用循环伏安法研究了2-巯基嘧啶（ MP）、4-氨基-2-巯基嘧啶（ AMP）及4，6-二氨基-2-巯基嘧啶（ DAMP）自组装修饰金电极的制备及其对细胞色素c直接电子传递的促进作用。用扫描电子显微镜（ SEM ）表征了2-巯基嘧啶衍生物修饰金电极的表面形貌。细胞色素c在MP、AMP修饰金电极上能进行准可逆的电化学反应，氧化还原峰电位差（ΔEp ）分别为61 mV和86 mV，氧化与还原峰电流比ipa/ipc接近1，峰电流与电位扫描速率平方根（v1/2）呈正比，是扩散控制的准可逆过程，异相电子迁移速率常数（Ks）分别为1.4×10-3 cm· s-1和5.1×10-5 cm· s-1。金电极在2.0 mmol· L-1 MP、AMP溶液中分别浸泡3 h和9 h，促进效果较好。随2-巯基嘧啶环上取代氨基数的增加，对细胞色素c电化学反应的促进作用及电极的稳定性逐渐减弱，即MP＞AMP，而DAMP基本无促进作用。
用循環伏安法研究瞭2-巰基嘧啶（ MP）、4-氨基-2-巰基嘧啶（ AMP）及4，6-二氨基-2-巰基嘧啶（ DAMP）自組裝脩飾金電極的製備及其對細胞色素c直接電子傳遞的促進作用。用掃描電子顯微鏡（ SEM ）錶徵瞭2-巰基嘧啶衍生物脩飾金電極的錶麵形貌。細胞色素c在MP、AMP脩飾金電極上能進行準可逆的電化學反應，氧化還原峰電位差（ΔEp ）分彆為61 mV和86 mV，氧化與還原峰電流比ipa/ipc接近1，峰電流與電位掃描速率平方根（v1/2）呈正比，是擴散控製的準可逆過程，異相電子遷移速率常數（Ks）分彆為1.4×10-3 cm· s-1和5.1×10-5 cm· s-1。金電極在2.0 mmol· L-1 MP、AMP溶液中分彆浸泡3 h和9 h，促進效果較好。隨2-巰基嘧啶環上取代氨基數的增加，對細胞色素c電化學反應的促進作用及電極的穩定性逐漸減弱，即MP＞AMP，而DAMP基本無促進作用。
용순배복안법연구료2-구기밀정（ MP）、4-안기-2-구기밀정（ AMP）급4，6-이안기-2-구기밀정（ DAMP）자조장수식금전겁적제비급기대세포색소c직접전자전체적촉진작용。용소묘전자현미경（ SEM ）표정료2-구기밀정연생물수식금전겁적표면형모。세포색소c재MP、AMP수식금전겁상능진행준가역적전화학반응，양화환원봉전위차（ΔEp ）분별위61 mV화86 mV，양화여환원봉전류비ipa/ipc접근1，봉전류여전위소묘속솔평방근（v1/2）정정비，시확산공제적준가역과정，이상전자천이속솔상수（Ks）분별위1.4×10-3 cm· s-1화5.1×10-5 cm· s-1。금전겁재2.0 mmol· L-1 MP、AMP용액중분별침포3 h화9 h，촉진효과교호。수2-구기밀정배상취대안기수적증가，대세포색소c전화학반응적촉진작용급전겁적은정성축점감약，즉MP＞AMP，이DAMP기본무촉진작용。
2-Mercaptopyrimidine ( MP),4-amino-2-mercaptopyrimidine ( AMP) and 4,6-diamino-2-mercaptopyrimidine ( DAMP) modified gold electrodes were prepared by self-assembled monolayer method ,and their promotion effects on the direct electron trans-fer of cytochrome c were investigated with cyclic voltammetry .The surface morphology of 2-mercaptopyrimidine derivative modified gold electrodes was characterized by scanning electron microscopy ( SEM) .The results shown that cytochrome c can carry out quasi-reversible electrochemical reaction on the MP and AMP modified gold electrodes ,the redox potential difference (ΔEp ) was 61 mV and 86 mV,respectively.The redox peak current ratio ipa/ipc was nearly 1.The peak current was positively correlated to the square root of potential scanning rate ( v1/2 ) ,indicating that it was a diffusion-controlled quasi-reversible process ,and the heterogeneous e-lectron migration rate constant(Ks)is 1.4×10-3cm· s-1 and 5.1×10-5 cm· s-1,respectively.The optimum promotion effect was ob-tained when the gold electrodes were immersed in 2.0 mmol· L-1 MP or AMP solution for 3 h or 9 h,respectively.The promotion and the stability of 2-mercaptopyrimidine derivatives modified electrodes to the direct electrochemistry of cytochrome c was decreased with the increasing number of amino groups in their molecular structure ,i.e.MP>AMP,but DAMP had almost no promotion .
