化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2013年
7期
934-939
,共6页
罗娅君%王照丽%罗利洪%张琦
囉婭君%王照麗%囉利洪%張琦
라아군%왕조려%라리홍%장기
牡荆提取物%N-二甲基亚硝胺%亚硝酸钠%阻断%清除
牡荊提取物%N-二甲基亞硝胺%亞硝痠鈉%阻斷%清除
모형제취물%N-이갑기아초알%아초산납%조단%청제
extract of vitex negundo L.var.cannabifolia%N-nitrosodimethylamine%sodium nitrite%inhibition%scavenging
采用正交试验法设计了从牡荆中提取活性物质的实验条件，研究其对亚硝化反应的抑制作用及对亚硝酸钠的清除作用。在体外模拟人胃液的条件下，用紫外-可见分光光度法测定了牡荆提取物对N-二甲基亚硝胺（ NDMA）体外合成的阻断作用及对亚硝酸钠的清除作用。结果表明：从牡荆中提取抗亚硝化反应活性物质的最佳条件是：料液比为1∶20，在80℃下用55％的甲醇水浴回流提取2 h。此提取物具有较高的阻断亚硝胺合成及清除亚硝酸钠的效果。其对亚硝胺合成的最大阻断率为88.24％，对亚硝酸钠的最大清除率为94.29％。
採用正交試驗法設計瞭從牡荊中提取活性物質的實驗條件，研究其對亞硝化反應的抑製作用及對亞硝痠鈉的清除作用。在體外模擬人胃液的條件下，用紫外-可見分光光度法測定瞭牡荊提取物對N-二甲基亞硝胺（ NDMA）體外閤成的阻斷作用及對亞硝痠鈉的清除作用。結果錶明：從牡荊中提取抗亞硝化反應活性物質的最佳條件是：料液比為1∶20，在80℃下用55％的甲醇水浴迴流提取2 h。此提取物具有較高的阻斷亞硝胺閤成及清除亞硝痠鈉的效果。其對亞硝胺閤成的最大阻斷率為88.24％，對亞硝痠鈉的最大清除率為94.29％。
채용정교시험법설계료종모형중제취활성물질적실험조건，연구기대아초화반응적억제작용급대아초산납적청제작용。재체외모의인위액적조건하，용자외-가견분광광도법측정료모형제취물대N-이갑기아초알（ NDMA）체외합성적조단작용급대아초산납적청제작용。결과표명：종모형중제취항아초화반응활성물질적최가조건시：료액비위1∶20，재80℃하용55％적갑순수욕회류제취2 h。차제취물구유교고적조단아초알합성급청제아초산납적효과。기대아초알합성적최대조단솔위88.24％，대아초산납적최대청제솔위94.29％。
The optimum conditions for extracting bioactive ingredients from Vitex negundo L.var.cannabifolia by Orthogonal Design , the inhibiting on nitrosation reaction and scavenging effects on sodium nitrite were studied .Under the conditions simulated according to human body’s gastric juice,the blocking rate to N-nitrosodimethylamine(NDMA)vitro synthesis and cleaning rate to sodium ni-trite by extract of vitex negundo L.var.cannabifolia were determined.The results show that the optimum extract conditions were as follows:concentration of methanol solution was 55%,the ratio of solid to solution was 1∶20 ,and extraction was carried out at 80 ℃for 2 hour.The extract has high capabilies on inhibiting nitrosamine synthesis and scavenging sodium nitrite .The maximum capabili-ties of inhibiting formation of nitrosamine and scavenging rate of sodium nitrite were 88.24%and 94.29%respectively.
