CAJ | 학술논문

近年来，以双向电泳技术、生物质谱和生物信息学为核心的蛋白质组学技术飞速发展，其大规模高通量的特点，使得这项技术逐渐在环境科学领域，尤其是对污染胁迫下差异蛋白表达和污染物特异性的生物标志物的筛选研究中得到了广泛的应用。本文简要介绍了蛋白质组学的基本概念和技术方法，对蛋白质组学在微生物、植物和动物对于无机污染物和有机污染物胁迫的响应机制和分子生物标志物探究等方面的研究进展进行了概述，并对蛋白质组学技术今后在生态环境领域的需求、应用与发展进行了展望。
근년래，이쌍향전영기술、생물질보화생물신식학위핵심적단백질조학기술비속발전，기대규모고통량적특점，사득저항기술축점재배경과학영역，우기시대오염협박하차이단백표체화오염물특이성적생물표지물적사선연구중득도료엄범적응용。본문간요개소료단백질조학적기본개념화기술방법，대단백질조학재미생물、식물화동물대우무궤오염물화유궤오염물협박적향응궤제화분자생물표지물탐구등방면적연구진전진행료개술，병대단백질조학기술금후재생태배경영역적수구、응용여발전진행료전망。
In recent years,the technology of proteomics has been rapidly developed with the three core techniques:Two-Di-mensional Electrophoresis,biological mass spectrometry and bioinformatics.Due to the large-scale and high-throughput fea-tures of these techniques,proteomics techniques have been increasingly applied to the field of environment science,especial-ly in the expression of differential proteins and the screening of pollutant-specific biomarkers under the pollution stress.The basic concepts and technologies are briefly introduced.And the requirement,applications and progress of proteomics in the responding mechanism to the inorganic and organic pollutants stress of microbe,plants and animals and the exploiting of the molecular biomarkers are reviewed.