化学传感器
化學傳感器
화학전감기
CHEMICAL SENSORS
2013年
3期
17-21
,共5页
王青%杨丽娟%羊小海%王柯敏%王辉
王青%楊麗娟%羊小海%王柯敏%王輝
왕청%양려연%양소해%왕가민%왕휘
纳米金颗粒%电化学生物传感%三聚氰胺
納米金顆粒%電化學生物傳感%三聚氰胺
납미금과립%전화학생물전감%삼취청알
Au nanoparticles%electrochemical biosensor%melamin
三聚氰胺与胸腺嘧啶（T）之间能够通过三个氢键结合，以富T的DNA探针为识别元件，结合DNA修饰的纳米金颗粒放大技术，以电活性物质钌胺作为信号分子，发展了一种高灵敏检测三聚氰胺的电化学传感器，该传感器具有良好的特异性和灵敏度，检测下限低至0.5 nmol/L。
三聚氰胺與胸腺嘧啶（T）之間能夠通過三箇氫鍵結閤，以富T的DNA探針為識彆元件，結閤DNA脩飾的納米金顆粒放大技術，以電活性物質釕胺作為信號分子，髮展瞭一種高靈敏檢測三聚氰胺的電化學傳感器，該傳感器具有良好的特異性和靈敏度，檢測下限低至0.5 nmol/L。
삼취청알여흉선밀정（T）지간능구통과삼개경건결합，이부T적DNA탐침위식별원건，결합DNA수식적납미금과립방대기술，이전활성물질조알작위신호분자，발전료일충고령민검측삼취청알적전화학전감기，해전감기구유량호적특이성화령민도，검측하한저지0.5 nmol/L。
Melamine could form triple H-bonds with thymine in aqueous medium. An electrochemical biosensor for melamine detection based on DNA modified gold nanoparticles amplification strategies was demonstrated. In this system, the T-rich DNA probes were immobilized on electrode surface as recognition element. The cationic electroactive complex [Ru(NH3)6]3+was bound to the DNA via electrostatic interactions as signal molecules. Since hundreds of DNA molecules were loaded on single gold nanoparticle, a good specificity and sensitivity was obtained in this method, the detection limit was ca. 0.5 nmol/L.
