CAJ | 학술논문

我国南北方海域均有浒苔 Enteromorpha sp.分布，但是迄今为止，鲜有关于南方海域浒苔的深入研究。对从大亚湾虾池中采集的浒苔进行了基于18S rDNA序列的分子鉴定，并研究了该浒苔对养殖废水中营养盐的吸收特性。18S RNA基因扩增及测序后获得长1154bp的序列，序列比对及种系发生分析结果表明，大亚湾浒苔与Ulva intestinalis 关系较近，与引起青岛“绿潮”的浒苔差异较大。对养殖废水中营养盐的吸收结果表明，利用该浒苔处理养殖废水的最优添加量为1.1g·L?1。在该添加量条件下，浒苔4h 能去除养殖废水中65%的 PO4-P、47%的NO2-N、91%的NO3-N、50%的NH4-N；8h对PO4-P的去除率达72%，对NO2-N的去除率为81%，对NO3-N的去除率为100%，对NH4-N的去除率为74%。因此，利用浒苔处理养殖废水具有时间短、效率高的特点，可用于富营养化水体或养殖废水的处理。研究可为海水养殖业的可持续发展提供一定的科学依据。
아국남북방해역균유호태 Enteromorpha sp.분포，단시흘금위지，선유관우남방해역호태적심입연구。대종대아만하지중채집적호태진행료기우18S rDNA서렬적분자감정，병연구료해호태대양식폐수중영양염적흡수특성。18S RNA기인확증급측서후획득장1154bp적서렬，서렬비대급충계발생분석결과표명，대아만호태여Ulva intestinalis 관계교근，여인기청도“록조”적호태차이교대。대양식폐수중영양염적흡수결과표명，이용해호태처리양식폐수적최우첨가량위1.1g·L?1。재해첨가량조건하，호태4h 능거제양식폐수중65%적 PO4-P、47%적NO2-N、91%적NO3-N、50%적NH4-N；8h대PO4-P적거제솔체72%，대NO2-N적거제솔위81%，대NO3-N적거제솔위100%，대NH4-N적거제솔위74%。인차，이용호태처리양식폐수구유시간단、효솔고적특점，가용우부영양화수체혹양식폐수적처리。연구가위해수양식업적가지속발전제공일정적과학의거。
The seaweed Enteromorpha sp. distributes widely in the coastal areas of China. There are, however, few studies on Enteromorpha in the South China Sea. In this study, we identified one Enteromorpha sp. isolated from Daya Bay using molecular method based on 18S rDNA sequence, and analyzed its nutrient (PO4-P, NO2-N, NO3-N, and NH4-N) removal capacity in aquaculture wastewater. The 18S rRNA gene sequence of Enteromorpha sp. was acquired with the length of 1154bp. Blast search and phylogenetic analysis showed that Enteromorpha sp. from Daya Bay was closely related to Ulva intestinalis and had large genetic distance with the species that caused the green tide in Qingdao in 2008. The results of nutrient absorption experiment indicated that the optimal treatment dose of Enteromorpha sp. for the wastewater was 1.1 g·L?1. Sixty-five percent of PO4-P, 47% of NO2-N, 91% of NO3-N, and 50% of NH4-N could be removed by the seaweed in four hours, and 72%of PO4-P, 81%of NO2-N, 100%of NO3-N, and 74%of NH4-N could be removed in eight hours. Therefore, it has advantage of high efficiency to use Enteromorpha to treat aquaculture wastewater. Enteromorpha can be a candidate for treating eutrophic water or aquaculture wastewater. This study could provide a scientific reference for the treatment and utilization of farming wastewater.