CAJ | 학술논문

目的：探讨靶向MDM2基因的反义寡核苷酸（ ASON）对MCF-7细胞株乳腺癌紫杉醇药物敏感性的影响。方法人工合成一段针对MDM2 mRNA的反义寡核苷酸及错义寡核苷酸，采用脂质体介导MDM2 ASON转染MCF-7乳腺癌细胞系，RT-PCR和Western blot方法检测其抑制效率，MTT观察细胞给药后的增殖能力。结果 MDM2 ASON转染细胞，给予紫杉醇处理后，MDM2 mRNA和蛋白表达下调，其中A500下调最显著，细胞增殖抑制率明显增高，其中A500抑制率高达（13.0±0.84）％。结论 ASON转染细胞后，下调MDM2的表达，促进凋亡，提高了对紫杉醇的敏感性，为乳腺癌治疗提供了新疗法。
목적：탐토파향MDM2기인적반의과핵감산（ ASON）대MCF-7세포주유선암자삼순약물민감성적영향。방법인공합성일단침대MDM2 mRNA적반의과핵감산급착의과핵감산，채용지질체개도MDM2 ASON전염MCF-7유선암세포계，RT-PCR화Western blot방법검측기억제효솔，MTT관찰세포급약후적증식능력。결과 MDM2 ASON전염세포，급여자삼순처리후，MDM2 mRNA화단백표체하조，기중A500하조최현저，세포증식억제솔명현증고，기중A500억제솔고체（13.0±0.84）％。결론 ASON전염세포후，하조MDM2적표체，촉진조망，제고료대자삼순적민감성，위유선암치료제공료신요법。
Objective To investigate the effects of the MDM2 antisense oligonucleotide ( ASON) on chemosensitivity to paclitaxel in human breast cancer cells MCF-7.Methods ASON and missense oligonucleotides were synthesized , MCF-7 cell lines was transfected by li-posome-mediated ASON , the inhibition efficiency was detected by RT-PCR and Western blot , proliferation of cells was observed by MTT .Results ASON transfected MDM2 mRNA and pro-tein expressions were significantly reduced after paclitaxel treatment , A500 was the most signif-icant, proliferation inhibition rate was significantly higher , and A500 inhibition rate was (13.0 ±0.84 )%.Conclusion ASON effectively suppresses the expression of MDM 2 mRNA, in-creases apoptosis and enhances the chemosensitivity to paclitaxel in MCF-7 , which provides a new treatment for breast cancer therapy .