CAJ | 학술논문

采用毒性试验方法,研究了安全浓度(1.288 mg/L)条件下亚砷酸钠(NaAsO2)对兰州鲇(Silurus lan-zhouensis)脑、鳃、肝脏、肌肉4种组织中6-磷酸葡萄糖脱氢酶(G-6-PDH)和乳酸脱氢酶(LDH)活性,以及RNA和蛋白质含量的影响。结果表明,染毒21d时, As(Ⅲ)可显著降低4种组织中G-6-PDH和LDH活性、RNA和蛋白质含量(P≤0.05)。撤毒后21d,除脑和肝组织中蛋白质含量未恢复到对照组水平(P≤0.05),肝脏中G-6-PDH活性超过了对照组水平(P≤0.05)外,其余各组织中 G-6-PDH和LDH活性、RNA和蛋白质含量均可恢复到对照组水平(P>0.05)。以上结果表明, As(Ⅲ)对兰州鲇组织中代谢酶活性具有明显的抑制作用,可致组织细胞RNA损伤和可溶性蛋白质减少,但这种影响是可逆的,撤毒后一定时间内可恢复到正常水平。
채용독성시험방법,연구료안전농도(1.288 mg/L)조건하아신산납(NaAsO2)대란주점(Silurus lan-zhouensis)뇌、새、간장、기육4충조직중6-린산포도당탈경매(G-6-PDH)화유산탈경매(LDH)활성,이급RNA화단백질함량적영향。결과표명,염독21d시, As(Ⅲ)가현저강저4충조직중G-6-PDH화LDH활성、RNA화단백질함량(P≤0.05)。철독후21d,제뇌화간조직중단백질함량미회복도대조조수평(P≤0.05),간장중G-6-PDH활성초과료대조조수평(P≤0.05)외,기여각조직중 G-6-PDH화LDH활성、RNA화단백질함량균가회복도대조조수평(P>0.05)。이상결과표명, As(Ⅲ)대란주점조직중대사매활성구유명현적억제작용,가치조직세포RNA손상화가용성단백질감소,단저충영향시가역적,철독후일정시간내가회복도정상수평。
The effects of Sodium arsenite on glucose-6-phosphate dehydrogenase (G-6-PDH) and lactate dehydrogenase (LDH) activities, RNA and protein contents in brain, gill, liver and muscle of Silurus lanzhouensis were investigated. The results indicated that during the 21d when the concentrations of Sodium arsenite were maintained safe in the water, G-6-PDH and LDH activities, RNA and protein contents decreased significantly in all tissues (P≤0.05). Then, remaining fish were transferred to clean water for the elimination phase of 21d. On the 21st day after moving, compared with the control level, G-6-PDH and LDH activities, RNA and protein contents in brain, gill, liver and muscle were restored to the control level (P>0.05), except for the lower protein contents in brain and liver and higher G-6-PDH activity in liver (P≤0.05). These results suggested that arsenic could inhibit metabolic activity, induce RNA damage and the content of soluble protein, and this process was reversible after withdraw arsenic.