中外医学研究
中外醫學研究
중외의학연구
CHINESE AND FOREIGN MEDICAL RESEARCH
2014年
14期
144-145
,共2页
宋莹%谷依学%仇秦威%贺智敏
宋瑩%穀依學%仇秦威%賀智敏
송형%곡의학%구진위%하지민
报告基因%ERCC1%启动子
報告基因%ERCC1%啟動子
보고기인%ERCC1%계동자
ERCC1%Luciferase reporter gene%Promoter
目的：构建人切除修复交叉互补基因1(excision repair cross complementation 1，ERCC1)启动子荧光素酶报告基因质粒。方法：以人基因组DNA为模板，扩增ERCC1启动子，将其重组到荧光素酶报告基因载体pGL4 Basic中，测序验证构建的质粒中包含ERCC1启动子序列。结果：测序结果正确，质粒pGL4-ERCC1-P1构建成功。结论：人ERCC1启动子荧光素酶报告基因质粒构建成功，可以用于后续基因表达调控的研究。
目的：構建人切除脩複交扠互補基因1(excision repair cross complementation 1，ERCC1)啟動子熒光素酶報告基因質粒。方法：以人基因組DNA為模闆，擴增ERCC1啟動子，將其重組到熒光素酶報告基因載體pGL4 Basic中，測序驗證構建的質粒中包含ERCC1啟動子序列。結果：測序結果正確，質粒pGL4-ERCC1-P1構建成功。結論：人ERCC1啟動子熒光素酶報告基因質粒構建成功，可以用于後續基因錶達調控的研究。
목적：구건인절제수복교차호보기인1(excision repair cross complementation 1，ERCC1)계동자형광소매보고기인질립。방법：이인기인조DNA위모판，확증ERCC1계동자，장기중조도형광소매보고기인재체pGL4 Basic중，측서험증구건적질립중포함ERCC1계동자서렬。결과：측서결과정학，질립pGL4-ERCC1-P1구건성공。결론：인ERCC1계동자형광소매보고기인질립구건성공，가이용우후속기인표체조공적연구。
Objective:To construct a human excision repair cross complementation 1(ERCC1) promoter luciferase reporter plasmid.Method:The ERCC1 promoter from human genomic DNA was amplified by PCR,and was inserted into the pGL4 Basic vector.The amplified DNA sequence was confirmed by sequencing.Result:DNA sequencing verified the successful construction of the plasmid pGL4-ERCC1-P1.Conclusion:The human ERCC1 promoter luciferase reporter gene vector is successfully constructed,which can be use for further study on gene expression regulation.
