酿酒
釀酒
양주
LIQUOR MAKING
2013年
6期
65-68
,共4页
李杨%李雷%任永利%李高凤%王继春
李楊%李雷%任永利%李高鳳%王繼春
리양%리뢰%임영리%리고봉%왕계춘
酒精酵母%耐酸%原生质体%紫外辐照%亚硝基胍%复合诱变
酒精酵母%耐痠%原生質體%紫外輻照%亞硝基胍%複閤誘變
주정효모%내산%원생질체%자외복조%아초기고%복합유변
alcohol yeast%acidproof%protoplast%ultraviolet radiation%DES%composite mutation
以耐酸性酒精酵母A3为出发菌株，对其原生质体进行紫外线（UV）与亚硝基胍（NTG）复合诱变，利用三级筛选模式，筛选出一株高产突变菌株UN1-81。该菌株在发酵结束后，酒精度达到11.17%vol，比出发菌株提高了16.47%以上，且经过20次传代培养，酒精产量稳定。
以耐痠性酒精酵母A3為齣髮菌株，對其原生質體進行紫外線（UV）與亞硝基胍（NTG）複閤誘變，利用三級篩選模式，篩選齣一株高產突變菌株UN1-81。該菌株在髮酵結束後，酒精度達到11.17%vol，比齣髮菌株提高瞭16.47%以上，且經過20次傳代培養，酒精產量穩定。
이내산성주정효모A3위출발균주，대기원생질체진행자외선（UV）여아초기고（NTG）복합유변，이용삼급사선모식，사선출일주고산돌변균주UN1-81。해균주재발효결속후，주정도체도11.17%vol，비출발균주제고료16.47%이상，차경과20차전대배양，주정산량은정。
In this experiment, acidproof alcohol yeast strain A3 was used as original strain and its protoplast was treated by UltraViolet(UV)-Nitrosoguanidine (NTG)composite mutation to obtain acid-proof and high ethanol-producing mutants. then a quality strain UN1-81 was finally screened by three-class screening mode from large quantity of flat plates. The mutated strain after fermentation could obtain 11.17%(Vol) alcohol concentration which enhanced 16.47 % to start strain, and the stable genotype with high ethanol yield had been verified through twenty times of transferring of culture.
