神经损伤与功能重建
神經損傷與功能重建
신경손상여공능중건
NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
2013年
6期
431-434
,共4页
赵秀秀%韩肖华%郭风%张靖慧%娄吉成%柳华%黄晓琳
趙秀秀%韓肖華%郭風%張靖慧%婁吉成%柳華%黃曉琳
조수수%한초화%곽풍%장정혜%루길성%류화%황효림
脑缺血%重复经颅磁刺激%cAMP反应元件结合蛋白%脑源性神经营养因子%血管内皮生长因子%巢蛋白
腦缺血%重複經顱磁刺激%cAMP反應元件結閤蛋白%腦源性神經營養因子%血管內皮生長因子%巢蛋白
뇌결혈%중복경로자자격%cAMP반응원건결합단백%뇌원성신경영양인자%혈관내피생장인자%소단백
cerebral ischemia%repetitive transcranial magnetic stimulation%cAMP response element binding protein%brain-derived neurotrophic factor%vascular endothelial growth factor%nestin
目的：研究高频重复经颅磁刺激（rTMS）对脑缺血大鼠缺血侧海马BDNF、VEGF和Nestin表达的影响。方法：SD雄性成年大鼠16只，随机分为模型组、rTMS组、rTMS＋生理盐水（NS）组和rTMS＋H89组各4只，rTMS＋NS组和rTMS＋H89组分别侧脑室注射NS和H89，4组均建立大脑中动脉栓塞（MCAO）模型，除模型组外均给予7 d 20 Hz rTMS治疗，应用Western Blot方法检测缺血侧海马pCREB、BDNF、VEGF和Nestin表达。结果：rTMS组pCREB、BDNF、VEGF和Nestin表达较模型组均增加（＜0.05），rTMS＋H89组pCREB、BDNF、VEGF和Nestin表达较rTMS＋NS组均减少（＜0.05）。结论：高频rTMS能使脑缺血后海马区BDNF、VEGF表达增加，并进一步促进Nestin表达，PKA-CREB通路的调节可能是其机制之一。
目的：研究高頻重複經顱磁刺激（rTMS）對腦缺血大鼠缺血側海馬BDNF、VEGF和Nestin錶達的影響。方法：SD雄性成年大鼠16隻，隨機分為模型組、rTMS組、rTMS＋生理鹽水（NS）組和rTMS＋H89組各4隻，rTMS＋NS組和rTMS＋H89組分彆側腦室註射NS和H89，4組均建立大腦中動脈栓塞（MCAO）模型，除模型組外均給予7 d 20 Hz rTMS治療，應用Western Blot方法檢測缺血側海馬pCREB、BDNF、VEGF和Nestin錶達。結果：rTMS組pCREB、BDNF、VEGF和Nestin錶達較模型組均增加（＜0.05），rTMS＋H89組pCREB、BDNF、VEGF和Nestin錶達較rTMS＋NS組均減少（＜0.05）。結論：高頻rTMS能使腦缺血後海馬區BDNF、VEGF錶達增加，併進一步促進Nestin錶達，PKA-CREB通路的調節可能是其機製之一。
목적：연구고빈중복경로자자격（rTMS）대뇌결혈대서결혈측해마BDNF、VEGF화Nestin표체적영향。방법：SD웅성성년대서16지，수궤분위모형조、rTMS조、rTMS＋생리염수（NS）조화rTMS＋H89조각4지，rTMS＋NS조화rTMS＋H89조분별측뇌실주사NS화H89，4조균건립대뇌중동맥전새（MCAO）모형，제모형조외균급여7 d 20 Hz rTMS치료，응용Western Blot방법검측결혈측해마pCREB、BDNF、VEGF화Nestin표체。결과：rTMS조pCREB、BDNF、VEGF화Nestin표체교모형조균증가（＜0.05），rTMS＋H89조pCREB、BDNF、VEGF화Nestin표체교rTMS＋NS조균감소（＜0.05）。결론：고빈rTMS능사뇌결혈후해마구BDNF、VEGF표체증가，병진일보촉진Nestin표체，PKA-CREB통로적조절가능시기궤제지일。
Objective: To investigate the effects of high-frequency repetitive transcranial magnetic stimulation (rTMS) on the expression of BDNF, VEGF and Nestin in the ischemic hippocampus of rats with cerebral is-chemia. Methods: A total of 16 SD rats were randomly divided into model group, rTMS group, rTMS+H89 group and rTMS+normal saline(NS) group (n=4 in each group). The rats in rTMS+H89 group and rTMS+NS group were intracerebroventricular injected with H89 or NS respectively. Reperfusion models with middle cere-bral artery occlusion were established in the 4 groups. rTMS of 20 Hz was given to 3 groups except model group for 7 days. The expression changes of BDNF, VEGF and Nestin were investigated between model group and rTMS group, rTMS+H89 group and rTMS+NS group by Western Blot respectively. Results:Compared with model control group,the expressions of pCREB, BDNF, VEGF and Nestin were increased in the rTMS group ( <0.05). Compared with rTMS+NS group , the expressions of pCREB, BDNF, VEGF and Nestin were signifi-cantly decreased in the rTMS+H89 group ( <0.05). Conclusion:Repetitive transcranial magnetic stimulation of 20Hz could promote BDNF and VEGF expressions in the ischemic hippocampus after cerebral ischemia, which may contribute to the neurogenesis.
