CAJ | 학술논문

目的探讨神经型钙黏蛋白(N-cadherin)分子在多发性骨髓瘤(MM)患者中的表达及其临床意义.方法收集54例初治MM患者及20名健康人骨髓单个核细胞,应用实时荧光定量PCR法检测N-cadherin mRNA表达,Western blot法测定其蛋白表达.结果 MM患者N-cadherin mRNA表达水平为6.056±3.033,健康对照组为1.788±0.778,二组相比差异有统计学意义(P＜0.0001).按照ISS分期,Ⅰ期、Ⅱ期、Ⅲ期MM患者N-cadherin mRNA表达水平分别为3.681±3.185、5.757±3.292、7.460±3.647,其中Ⅱ期、Ⅲ期与健康对照组相比差异均具有统计学意义(均P＜0.05);Ⅰ期与健康对照组相比差异无统计学意义(P＞0.05).有髓外浸润及无髓外浸润患者N-cadherin mRNA表达量分别为9.159±3.178、5.083±3.288,差异有统计学意义(P＜ 0.001).有骨质破坏和无骨质破坏患者N-cadherin mRNA表达量分别为6.544±3.729、4.240±2.896,差异有统计学意义(P=0.047).初治MM患者N-cadherin mRNA表达水平为6.056±3.033,治疗后有效患者N-cadherin mRNA表达水平下降(3.768±2.216)(P=0.015),治疗无效患者N-cadherin mRNA水平与治疗前相比差异无统计学意义(P＞0.05).初治MM患者N-cadherin蛋白表达量明显高于缓解期患者及健康对照组,而在治疗后复发的患者中,N-cadherin蛋白表达量明显高于初治患者.结论 N-cadherin分子在MM患者中高表达,其可能参与MM的发生及发展.
목적 탐토신경형개점단백(N-cadherin)분자재다발성골수류(MM)환자중적표체급기림상의의.방법 수집54례초치MM환자급20명건강인골수단개핵세포,응용실시형광정량PCR법검측N-cadherin mRNA표체,Western blot법측정기단백표체.결과 MM환자N-cadherin mRNA표체수평위6.056±3.033,건강대조조위1.788±0.778,이조상비차이유통계학의의(P＜0.0001).안조ISS분기,Ⅰ기、Ⅱ기、Ⅲ기MM환자N-cadherin mRNA표체수평분별위3.681±3.185、5.757±3.292、7.460±3.647,기중Ⅱ기、Ⅲ기여건강대조조상비차이균구유통계학의의(균P＜0.05);Ⅰ기여건강대조조상비차이무통계학의의(P＞0.05).유수외침윤급무수외침윤환자N-cadherin mRNA표체량분별위9.159±3.178、5.083±3.288,차이유통계학의의(P＜ 0.001).유골질파배화무골질파배환자N-cadherin mRNA표체량분별위6.544±3.729、4.240±2.896,차이유통계학의의(P=0.047).초치MM환자N-cadherin mRNA표체수평위6.056±3.033,치료후유효환자N-cadherin mRNA표체수평하강(3.768±2.216)(P=0.015),치료무효환자N-cadherin mRNA수평여치료전상비차이무통계학의의(P＞0.05).초치MM환자N-cadherin단백표체량명현고우완해기환자급건강대조조,이재치료후복발적환자중,N-cadherin단백표체량명현고우초치환자.결론 N-cadherin분자재MM환자중고표체,기가능삼여MM적발생급발전.
Objective To investigate the expression and clinical significance of N-cadherin in patients with multiple myeloma (MM).Methods A total of newly diagnosed 54 MM patients and 20 controls were enrolled in this study.The expression of N-cadherin was detected by quantitative real-time polymerase chain reaction and Western blot.Results The N-cadherin mRNA expression levels of MM patients and normal controls were 6.056±3.033 and 1.788±0.778 (P ＜ 0.000 1).N-cadherin mRNA expression levels of ISS stage Ⅰ,Ⅱ and Ⅲ were 3.681±3.185,5.757±3.292 and 7.460±3.647,respectively.The results indicated that N-cadherin was significantly up-regulated in stage Ⅱ and stage Ⅲ (P ＜ 0.05),not in stage Ⅰ (P ＞ 0.05) compared with controls.The median levels of N-cadherin mRNA expression in MM patients with or without extramedullary infiltration were 9.159±3.178 and 5.083±3.288,respectively (P ＜ 0.001).MM patients with bone destruction had a higher N-cadherin mRNA expression level (6.544±3.729) than patients without bone destruction (4.240±2.896) (P =0.047).N-cadherin mRNA expression in newly diagnosed MM patients was 6.056±3.033,the expression in responded patients after treatment was significantly decreased (3.768±2.216) (P =0.015),and in no responded patients the levels of N-cadherin were not significantly decreased (P ＞ 0.05).The levels of N-cadherin protein were up-regulated in newly diagnosed patients and relapsed patients compared with controls,which was reduced in remission MM patients.And relapsed patients had a higher N-cadherin protein expression than newly diagnosed patients.Conclusion High expression of N-cadherin in MM patients may play an important role during the occurrence and development of MM.