CAJ | 학술논문

目的：从分子水平揭示前列腺癌骨转移的发病机制，为临床诊疗提供新思路。方法在公共基因芯片数据库（GEO）中下载前列腺癌骨转移的相关基因芯片数据，利用BRB-ArrayTools 软件、STRING、ToppGene、GOEAST、DAVID等生物信息学工具进行数据挖掘及生物信息学分析。结果 BRB分析筛选出501个前列腺癌骨转移差异基因，其中上调181个，下调320个。对其进行生物信息学分析发现SPP1、HBB、AR、MMP9、AZGP1、POSTN、FN1、VCAN等基因以及胶原蛋白及其生物合成、细胞黏附、小分子代谢过程、黏着斑、ECM受体相互作用、细胞周期、整合素信号通路等分子生物学过程及通路在前列腺癌骨转移的发生发展中可能起着重要作用。结论利用生物信息学的方法能有效分析基因芯片数据，并获取生物内在信息，为发现前列腺癌骨转移的早期诊断标志与治疗靶点提供新的思路。
목적：종분자수평게시전렬선암골전이적발병궤제，위림상진료제공신사로。방법재공공기인심편수거고（GEO）중하재전렬선암골전이적상관기인심편수거，이용BRB-ArrayTools 연건、STRING、ToppGene、GOEAST、DAVID등생물신식학공구진행수거알굴급생물신식학분석。결과 BRB분석사선출501개전렬선암골전이차이기인，기중상조181개，하조320개。대기진행생물신식학분석발현SPP1、HBB、AR、MMP9、AZGP1、POSTN、FN1、VCAN등기인이급효원단백급기생물합성、세포점부、소분자대사과정、점착반、ECM수체상호작용、세포주기、정합소신호통로등분자생물학과정급통로재전렬선암골전이적발생발전중가능기착중요작용。결론이용생물신식학적방법능유효분석기인심편수거，병획취생물내재신식，위발현전렬선암골전이적조기진단표지여치료파점제공신적사로。
Objective To better understand the molecular pathogenesis of prostate cancer bone metastasis, and provide novel approaching for clinical diagnosis and treatment of this malignancy. Methods The data of whole genomic expression profiles on prostate cancer bone metastasis were obtained from GEO database.A set of bioinformatics tools, such as BRB-ArrayTools, STRING, ToppGene, GOEAST and DAVID softwares were used to accomplish the data-mining and bioinformatics analysis. Results BRB analysis results showed there were 501 differentially expressed genes related to prostate cancer bone metastasis, including 181 up-regulated and 320 down-regulated. Bioinformatic analysis results suggested that SPP1, HBB, AR, MMP9, AZGP1, POSTN, FN1 and VCAN played essential roles in such important biological processes as collagen biosynthetic,cell adhesion,Focal adhesion,Integrin signalling pathway and ECM-receptor interaction. Conclusion Bioinformatic analysis had a high efficiency in analyzing gene chip data and revealing internal biology information. It will offer a new view to find early biomarkers and treatment targets of prostate cancer bone metastasis.