临床儿科杂志
臨床兒科雜誌
림상인과잡지
2013年
10期
949-952
,共4页
孙瑾%韩波%陈永芬%魏美丽
孫瑾%韓波%陳永芬%魏美麗
손근%한파%진영분%위미려
病毒性心肌炎%半乳糖凝集素-1%小鼠
病毒性心肌炎%半乳糖凝集素-1%小鼠
병독성심기염%반유당응집소-1%소서
viral myocarditis%galectin-1%mouse
目的：探讨半乳糖凝集素-1（galectin-1）在病毒性心肌炎（VMC）小鼠心肌细胞中表达的动态变化。方法选取雄性BALB/c小鼠58只，随机分为病毒组50只、对照组8只。病毒组小鼠腹腔接种TCID50为10-5/L的Nancy株CVB3病毒液0.10 ml，并于接种后第7、10、14和28天，分别随机处死8只小鼠；对照组小鼠腹腔接种不含病毒eagle液0.10 ml，于接种后第28天处死。光镜下观察小鼠心肌组织的病理变化，ELISA法检测外周血中galectin-1水平，实时荧光定量聚合酶链反应（RQ-PCR）检测心肌组织中galectin-1 mRNA的表达。结果与对照组比较，病毒组小鼠心肌组织galectin-1 mRNA的表达在第7、10天均增高，差异均有统计学意义（P均<0.05）；病毒组小鼠外周血galectin-1水平在第7天、10、14天亦增高，差异均有统计学意义（P均<0.05）。结论 VMC小鼠心肌细胞中galectin-1的表达呈动态变化，galectin-1在VMC的发生发展中可能起一定作用。
目的：探討半乳糖凝集素-1（galectin-1）在病毒性心肌炎（VMC）小鼠心肌細胞中錶達的動態變化。方法選取雄性BALB/c小鼠58隻，隨機分為病毒組50隻、對照組8隻。病毒組小鼠腹腔接種TCID50為10-5/L的Nancy株CVB3病毒液0.10 ml，併于接種後第7、10、14和28天，分彆隨機處死8隻小鼠；對照組小鼠腹腔接種不含病毒eagle液0.10 ml，于接種後第28天處死。光鏡下觀察小鼠心肌組織的病理變化，ELISA法檢測外週血中galectin-1水平，實時熒光定量聚閤酶鏈反應（RQ-PCR）檢測心肌組織中galectin-1 mRNA的錶達。結果與對照組比較，病毒組小鼠心肌組織galectin-1 mRNA的錶達在第7、10天均增高，差異均有統計學意義（P均<0.05）；病毒組小鼠外週血galectin-1水平在第7天、10、14天亦增高，差異均有統計學意義（P均<0.05）。結論 VMC小鼠心肌細胞中galectin-1的錶達呈動態變化，galectin-1在VMC的髮生髮展中可能起一定作用。
목적：탐토반유당응집소-1（galectin-1）재병독성심기염（VMC）소서심기세포중표체적동태변화。방법선취웅성BALB/c소서58지，수궤분위병독조50지、대조조8지。병독조소서복강접충TCID50위10-5/L적Nancy주CVB3병독액0.10 ml，병우접충후제7、10、14화28천，분별수궤처사8지소서；대조조소서복강접충불함병독eagle액0.10 ml，우접충후제28천처사。광경하관찰소서심기조직적병리변화，ELISA법검측외주혈중galectin-1수평，실시형광정량취합매련반응（RQ-PCR）검측심기조직중galectin-1 mRNA적표체。결과여대조조비교，병독조소서심기조직galectin-1 mRNA적표체재제7、10천균증고，차이균유통계학의의（P균<0.05）；병독조소서외주혈galectin-1수평재제7천、10、14천역증고，차이균유통계학의의（P균<0.05）。결론 VMC소서심기세포중galectin-1적표체정동태변화，galectin-1재VMC적발생발전중가능기일정작용。
Objectives To investigate the dynamic change of galectin-1 in myocardium of mice with viral myocarditis (VMC). Methods A total of 58 male BALB/c mice were selected and randomly divided into CVB3 group (n=50) and control group (n=8). Mice in CVB3 group were infected with 0.10 ml 10-5/L CVB3 through intraperitoneal inoculation, and 8 mice were killed on day 7, 10, 14 and 28 respectively after inoculation. Mice in control group inoculated with 0.10 ml eagle reagent was killed on day 28. The myocardial pathological changes were observed using light microscope. In addition, expressions of serum galectin-1 were detected by ELISA and expressions of myocardial galectin-1 were detected by real-time quantitative-polymerase chain reaction (RQ-PCR). Results Compared with control group, the myocardial expression of galectin-1 mRNA in CVB3 group was obviously increased on day 7 and day 10 (all P<0.05);the serum concentration of galectin-1 in CVB3 group was obviously increased on day 7, day 10 and day 14 (all P<0.05). Conclusion The expressions of galectin-1 in myocardium of mice with viral myocarditis were in dynamic changes. Galectin-1 may play a role in the pathogenesis and development of viral myocarditis.
