CAJ | 학술논문

目的：探讨儿童急性白血病（AL）患儿胰岛素样生长因子表达的变化。方法选择初治AL患儿50例，以同期健康体检儿童30例为对照组。AL患儿按化疗方案正规序贯化疗，均未接受颅脑放射治疗。AL患儿分别在治疗前及完全缓解后6个月，应用酶联免疫分析法测定血清血清胰岛素样生长因子1（IGF-1）、游离胰岛素样生长因子1（f IGF-1）、胰岛素样生长因子结合蛋白3（IGFBP-3）水平，并与对照组儿童比较。结果治疗前，AL患儿血清IGF-1、IGFBP-3水平均低于对照组；fIGF-1水平高于对照组，差异均有统计学意义（P<0.01）。在治疗完全缓解后6个月，AL患儿血清IGF-1和fIGF-1水平与治疗前差异无统计学意义（P均>0.05），而与对照组差异仍有统计学意义（P<0.05）；IGFBP-3水平与治疗前差异有统计学意义（P<0.01），与对照组差异无统计学意义（P>0.05）。治疗前，AL患儿血清IGFBP-3水平与IGF-1呈正相关（r=0.777， P<0.01），与fIGF-1呈负相关（r=-0.714，P<0.01）。结论胰岛素样生长因子可能参与儿童AL的病理生理过程。
목적：탐토인동급성백혈병（AL）환인이도소양생장인자표체적변화。방법선택초치AL환인50례，이동기건강체검인동30례위대조조。AL환인안화료방안정규서관화료，균미접수로뇌방사치료。AL환인분별재치료전급완전완해후6개월，응용매련면역분석법측정혈청혈청이도소양생장인자1（IGF-1）、유리이도소양생장인자1（f IGF-1）、이도소양생장인자결합단백3（IGFBP-3）수평，병여대조조인동비교。결과치료전，AL환인혈청IGF-1、IGFBP-3수평균저우대조조；fIGF-1수평고우대조조，차이균유통계학의의（P<0.01）。재치료완전완해후6개월，AL환인혈청IGF-1화fIGF-1수평여치료전차이무통계학의의（P균>0.05），이여대조조차이잉유통계학의의（P<0.05）；IGFBP-3수평여치료전차이유통계학의의（P<0.01），여대조조차이무통계학의의（P>0.05）。치료전，AL환인혈청IGFBP-3수평여IGF-1정정상관（r=0.777， P<0.01），여fIGF-1정부상관（r=-0.714，P<0.01）。결론이도소양생장인자가능삼여인동AL적병리생리과정。
Objective To detect the levels of insulin-like growth factors in children with acute leukemia (AL). Methods A total of 50 previously untreated AL patients were selected, meanwhile 30 healthy children were selected as normal controls. AL children were given regular chemotherapy. All cases were not given the brain radiotherapy. The levels of insulin-like growth factor-1 (IGF-1), free insulin-like growth factor-1 (fIGF-1), insulin-like growth factor binding protein 3 (IGFBP-3) in AL patients before treatment and 6 months after complete remission were measured by enzyme-linked immunosorbent assay (ELISA), and were compared with those in normal controls. Results Before treatment, compared with normal controls, the serum levels of IGF-1, IGFBP-3 in AL patients were lower while the level of fIGF-1 was higher, and the differences were signiifcant (P<0.01). At six months after complete remission, the levels of IGF-1 and fIGF-1 in AL patients were similar to those before treatment, but were signiifcantly different from those in control group (P<0.05);the level of IGFBP-3 was signiifcantly higher than that before treatment (P<0.01), but was similar to that in control group. Before treatment, the level of IGFBP-3 in AL patients was positively correlated with the level of IGF-1 (r=0.777, P<0.01), and negatively correlated with the level of fIGF-1 (r=-0.714, P<0.01). Conclusion Insuline-like growth factors were involved in the pathophysiological process in children with AL.