CAJ | 학술논문

目的：探讨miR-26b过表达在人脂肪细胞分化过程中对各种脂因子分泌的影响。方法以miR-26b过表达病毒感染人脂肪前体细胞并诱导分化；收集不同时间点的细胞培养基上清，采用酶联免疫吸附法（ELISA）检测各个时间点的脂因子（白介素-6、瘦素、抵抗素、肿瘤坏死因子-α）分泌量。结果与对照组比较，在miR-26b过表达病毒感染的人脂肪前体细胞分化过程中，白介素-6、瘦素分泌量降低，而抵抗素分泌量升高；两组均未检测到肿瘤坏死因子-α。结论 miR-26b在一定程度上可以改善人脂肪细胞炎症状态及改善胰岛素抵抗，为肥胖治疗提供潜在靶点。[临床儿科杂志,2013,31(10)：914-916]
목적：탐토miR-26b과표체재인지방세포분화과정중대각충지인자분비적영향。방법이miR-26b과표체병독감염인지방전체세포병유도분화；수집불동시간점적세포배양기상청，채용매련면역흡부법（ELISA）검측각개시간점적지인자（백개소-6、수소、저항소、종류배사인자-α）분비량。결과여대조조비교，재miR-26b과표체병독감염적인지방전체세포분화과정중，백개소-6、수소분비량강저，이저항소분비량승고；량조균미검측도종류배사인자-α。결론 miR-26b재일정정도상가이개선인지방세포염증상태급개선이도소저항，위비반치료제공잠재파점。[림상인과잡지,2013,31(10)：914-916]
Objective To investigate the inlfuence of overexpression of miR-26b on the secretion of adipokines dur-ing human adipocyte differentiation. Methods Human preadipocytes were infected with the hsa-miR-26b over-expressing lentivirus and were induced to differentiate, and then the levels of adipokines (IL-6, leptin, resistin, TNF-α) at different time points during differentiation were measured by ELISA. Results Compared with control group, decreased secretions of both IL-6 and leptin, and increased secretion of resistin were found during the differentiation of human adipocytes in miR-26b overexpressed group. However, the secretion of TNF-αwas not measured in both groups. Conclusion The miR-26b can improve the inlfammation and insulin resistance of human adipocytes, which will provide potential targets for obesity treat-ment.