CAJ | 학술논문

利用 Tyl-copia类逆转座子逆转录酶（ RT）的保守区域设计引物，优化了扩增条件后利用RT-PCR从白桦愈伤组织中得到了1条逆转录酶序列。利用硝普钠（ SNP）和茉莉酸甲酯（ MeJA）诱导后分别扩增克隆了11条和9条逆转录酶序列。这21条逆转录酶序列（命名Bprt1-21）变化范围为205～290 bp，一致性为71．93％，异质性为11．％～50．9％，翻译成氨基酸的异质性为0～57．1％。经SNP和MeJA处理的样品中，获得的逆转录酶序列基本是分别聚类，初步证明不同的Ty1-copia类逆转座子能够分别响应不同的信号而被转录激活。分析所获得的21逆转录酶序列和苹果中19条逆转录转座子序列（ DQ410748～DQ410766）进化关系发现，逆转录酶基本各自聚类，但是Bprt9、Bprt11和DQ410750进化关系比较接近，Bprt15、Bprt19、DQ410751和DQ410753聚为一类，证明逆转座子存在水平传递。
이용 Tyl-copia류역전좌자역전록매（ RT）적보수구역설계인물，우화료확증조건후이용RT-PCR종백화유상조직중득도료1조역전록매서렬。이용초보납（ SNP）화말리산갑지（ MeJA）유도후분별확증극륭료11조화9조역전록매서렬。저21조역전록매서렬（명명Bprt1-21）변화범위위205～290 bp，일치성위71．93％，이질성위11．％～50．9％，번역성안기산적이질성위0～57．1％。경SNP화MeJA처리적양품중，획득적역전록매서렬기본시분별취류，초보증명불동적Ty1-copia류역전좌자능구분별향응불동적신호이피전록격활。분석소획득적21역전록매서렬화평과중19조역전록전좌자서렬（ DQ410748～DQ410766）진화관계발현，역전록매기본각자취류，단시Bprt9、Bprt11화DQ410750진화관계비교접근，Bprt15、Bprt19、DQ410751화DQ410753취위일류，증명역전좌자존재수평전체。
We designed the primers of reverse transcriptase according to the conserved domains of the Ty1-copia retrotransposons reverse transcriptase.We obtained several fragments of reverse transcriptase from callus of birch by using improved RT-PCR.Eleven and nine fragments were amplified from callus of birch treated by sodium nitroprusside ( SNP) and methyl jasmonate (MeJA), respectively.The length of 21 reverse transcriptase sequences (named Bprt 1-21) is from 205 to 290 bp, with the consistency of 71.93%, the heterogeneity of from 1.1% to 50.9% and the heterogeneity of amino acid se-quences of from 0.0% to 57.1%.The sequences from birch treated by SNP or MeJA are clustered, respectively.The transcription of Ty1-copia-like retrotransposons can be activated by different signals.The analysis of the phylogenetic tree of reverse transcriptase of Ty1-coip a-like retrotransposons between birch and apple revealed each of the reverse transcripta-ses are clustered together, but the evolutional relationship between Bprt9, Bprt11 and DQ410750 are closer.Bprt15, Bprt19, DQ410751 and DQ410753 are clustered together.Therefore, the horizontal transmission can exist in retrotrans-posons.