CAJ | 학술논문

为观察华蟾毒精（CBG）对小鼠免疫细胞活性的影响，本研究采用MTT法检测小鼠脾淋巴细胞的增殖情况，检测小鼠腹腔巨噬细胞的吞噬功能以及小鼠NK细胞对靶细胞的杀伤作用。结果显示，CBG在一定剂量范围内单独或者协同非特异性丝裂原（Con A或LPS）作用能够显著增强小鼠脾淋巴细胞的增殖，CBG单独作用可以显著提高小鼠腹腔巨噬细胞的吞噬功能，并能够显著提高小鼠NK细胞对靶细胞的杀伤作用，表明CBG能够提高小鼠免疫细胞的活性。
위관찰화섬독정（CBG）대소서면역세포활성적영향，본연구채용MTT법검측소서비림파세포적증식정황，검측소서복강거서세포적탄서공능이급소서NK세포대파세포적살상작용。결과현시，CBG재일정제량범위내단독혹자협동비특이성사렬원（Con A혹LPS）작용능구현저증강소서비림파세포적증식，CBG단독작용가이현저제고소서복강거서세포적탄서공능，병능구현저제고소서NK세포대파세포적살상작용，표명CBG능구제고소서면역세포적활성。
In order to observe the effect of cinobufagin (CBG) on the activity of immune cells in mice, MTT method was used to detect the proliferation of splenic lymphocytes, the phagocytosis of peri-toneal macrophages and the killing ability of the NK cells to target cells in mice. The results showed that CBG within the specific dose can enhance the proliferation of mice splenic lymphocytes alone or work with non-specific mitogen (Con A or LPS), CBG alone can enhance the phagocytosis ability of perito-neal macrophages and raise the killing ability of NK cell to target cell remarkably. The results demon-strated that CBG can enhance the activity of immune cells in mice.