CAJ | 학술논문

猪细小病毒毒株在不同细胞系上的生长情况不尽相同，如不能掌握其规律，将难以生产出优质的疫苗。本研究对PPV (M2株)在PK15和ST细胞上生长情况进行了研究，得出ST细胞更适合其生长。之后将PPV M2毒株同步接种于ST细胞，用测定HA和TCID50的方法研究了其在ST细胞上增殖的基本规律。病毒在接种后24h可在细胞较稀的区域看到非常轻微的病变，病毒TCID50测定结果也表明，接毒后17h，病毒已经明显增殖。当病毒培养到约40h，其TCID50即接近高峰，并进入平台期，病毒的TCID50已达到107.5/0.1mL以上。继续培养，最高可达到108.5/0.1mL以上，且直到122h，也不见明显降低。病毒培养40~122h，不管是用Gibco血清还是用Hyclone血清培养病毒，其差异都非常小，变异系数都在5%以内。病毒HA价也出现同样的平台期，但HA价的平台期出现比TCID50的平台期出现更晚。
저세소병독독주재불동세포계상적생장정황불진상동，여불능장악기규률，장난이생산출우질적역묘。본연구대PPV (M2주)재PK15화ST세포상생장정황진행료연구，득출ST세포경괄합기생장。지후장PPV M2독주동보접충우ST세포，용측정HA화TCID50적방법연구료기재ST세포상증식적기본규률。병독재접충후24h가재세포교희적구역간도비상경미적병변，병독TCID50측정결과야표명，접독후17h，병독이경명현증식。당병독배양도약40h，기TCID50즉접근고봉，병진입평태기，병독적TCID50이체도107.5/0.1mL이상。계속배양，최고가체도108.5/0.1mL이상，차직도122h，야불견명현강저。병독배양40~122h，불관시용Gibco혈청환시용Hyclone혈청배양병독，기차이도비상소，변이계수도재5%이내。병독HA개야출현동양적평태기，단HA개적평태기출현비TCID50적평태기출현경만。
The growth property of one PPV strain in different cell lines were variable and the related knowledge is important for preparation of high-quality PPV vaccines. In this paper, we found that the ST cell was better for PPV M2 strain proliferation after comparing its proliferation profile in PK15 and ST cell lines. Then, the growth property of porcine parvovirus M2 strain in ST cells was studied by test it's TCID50 and HA test. The results indicated that slight CPE could be observed in 17h post inoculation. The TCID50 was near to the peak about 40h after porcine parvovirus M2 strain inoculation (above 107.5/0.1mL), and soon reached a plateau phase. The highest TCID50 could reach 108.5/0.1mL. No obvious dropping of TCID50 could be observed, even until 122h post inoculation. Within 40~122h after inoculation, whether cultured using FBS from Gibco or Hyclone, the TCID50 CV was below 5%. The HA also rose with the culture time and reached a plateau phase soon until 50h.