CAJ | 학술논문

2012年采用PCR-DGGE法对云南省典型高原湿地---剑湖地区的典型水生植物茭草的根际土壤固氮菌多样性进行分析。结果显示，入水口永丰河的茭草根际土壤固氮菌Shannon-Wiener指数和Simpson丰富度最高，出水口黑惠河的茭草根际土壤固氮菌Shannon-Wiener 指数和Simpson丰富度最低。典型对应分析表明， NH4＋和NO3-显著影响根际土壤固氮菌群落结构。同源比对分析显示，所有序列共分为2个类群，其中有11条带属于Betaproteobacteria ，占84.6％；2条带属于Gammaproteobacteria ，占15.3％。聚类分析结果表明，大部分固氮菌（84.6％）属于红环菌目，少部分（15.3％）属于着色菌目。
2012년채용PCR-DGGE법대운남성전형고원습지---검호지구적전형수생식물교초적근제토양고담균다양성진행분석。결과현시，입수구영봉하적교초근제토양고담균Shannon-Wiener지수화Simpson봉부도최고，출수구흑혜하적교초근제토양고담균Shannon-Wiener 지수화Simpson봉부도최저。전형대응분석표명， NH4＋화NO3-현저영향근제토양고담균군락결구。동원비대분석현시，소유서렬공분위2개류군，기중유11조대속우Betaproteobacteria ，점84.6％；2조대속우Gammaproteobacteria ，점15.3％。취류분석결과표명，대부분고담균（84.6％）속우홍배균목，소부분（15.3％）속우착색균목。
The diversity and community structure of nitrogen-fixing bacteria in the rhizosphere soil of Zizania ca-duciflora roots were investigated by using denaturing gradient gel electrophoresis ( DGGE ) . The results showed that Shannon-Wiener index and simpson index nitrogen-fixing bacteria of rhizosphere soil of Zizania caduciflora in Yongfeng river were the highest , while the lowest index were found in Heihui river .The result of canonical corre-spondence analysis of the relationships between nitrogen-fixing bacteria and soil chemical parameters showed that NH4 +and NO3 -had an significiant impact on nitrogen-fixing bacteria community.The sequence comparison anal-ysis showed that all sequences could be divided into two groups , namely Betaproteobacteria and Gammaproteobac-teria, and among these, 11 belongs to Betaproteobacteria ( accounts for 84.6 %), and 2 blongs Gammapro-teobacteria (accounts for 15.3 %).In addition, The result of cluster analysis based on DGGE sequences showed that most nitrogen-fixing bacteria belongs to Rhodocyclales ( accounts for 84.6%) , and only small portion belongs to Chromatiales (15.3 %).