CAJ | 학술논문

以云南省香格里拉县4个滇牡丹天然居群为研究对象，采用ISSR分子标记技术，对98份材料进行遗传多样性分析，用多态性高、稳定性强的10条引物进行扩增，共获得96条扩增产物，其中多态性条带有82条，多态性百分比为85.42％；滇牡丹总体基因多样性为0.3438， Shannon 指数为0.5009；通过Nei’ s和聚类分析，居群间的遗传分化系数为0.7973，居群间的遗传变异占总遗传变异的79.73％，而居群内的遗传变异只有20.27％，表明滇牡丹居群间的遗传分化较大，遗传变异主要存在于居群间。因此，滇牡丹虽然为分布区狭窄的特有种，但是与一些典型稀有和濒危的物种相比，遗传多样性并不低，滇牡丹的分布区域和种群数量呈现狭窄化、缩小化的趋势，主要原因是受人为过度采挖，生境受到破坏所致。
이운남성향격리랍현4개전모단천연거군위연구대상，채용ISSR분자표기기술，대98빈재료진행유전다양성분석，용다태성고、은정성강적10조인물진행확증，공획득96조확증산물，기중다태성조대유82조，다태성백분비위85.42％；전모단총체기인다양성위0.3438， Shannon 지수위0.5009；통과Nei’ s화취류분석，거군간적유전분화계수위0.7973，거군간적유전변이점총유전변이적79.73％，이거군내적유전변이지유20.27％，표명전모단거군간적유전분화교대，유전변이주요존재우거군간。인차，전모단수연위분포구협착적특유충，단시여일사전형희유화빈위적물충상비，유전다양성병불저，전모단적분포구역화충군수량정현협착화、축소화적추세，주요원인시수인위과도채알，생경수도파배소치。
ISSR molecular marker technology was used to analyze genetic diversity of 4 natural populations of 98 Paeonia delavayi individuals from shangri-la in Yunnan .10 primers with high polymorphism and steady expansion were selected and 96 bands were identified , of which 82 bands were polymorphic , the polymorphism percentage was 85.42 %.The total genetic diversity of 98 Paeonia delavayi was 0.343 8, and the Shannon index was 0.500 9 .Through the Nei ’ s and Cluster analysis , the gene differentiation coefficient among populations was 0.797 3, the genetic variation among populations was 79.73%, the genetic variation within populations was only 20.27 %.All of these indicates that the different populations of Paeonia delavayi had a larger genetic differentia-tion, and the main genetic variation was existed among populations .The study also showed that although Paeonia delavayi was an endemic species , compared with some typical rare and endangered species , its genetic diversity was not low .The reason of its narrowing distribution tendency was mainly caused by over collection and habitat dis -turbance from human activities .