CAJ | 학술논문

目的：探讨N（2）-L-丙氨酰-L-谷氨酰胺（丙谷二肽）对γ射线致小鼠肺组织损伤的防护作用及可能的作用机制。方法：将27只雄性昆明小鼠随机分为正常对照组、辐射组和丙谷二肽组，每组9只。丙谷二肽组按照1000 mg/kg的剂量灌胃给药，1次/d，连续7 d。然后辐射组、丙谷二肽组均给予4．0 Gy 60 Coγ射线照射3 d，正常对照组不作处理。照射后测定小鼠肺脏指数、肺组织超氧化物歧化酶（ SOD）活性，MTT法测定肺组织细胞增殖率， AnnexinV和PI双染法测定细胞凋亡率，Western blot法检测肺组织Bcl-2和Bax蛋白的表达。结果：3组小鼠肺脏指数、肺组织SOD活性、细胞凋亡率、肺组织Bcl-2和Bax蛋白表达水平差异均有统计学意义（ F＝6．352、51．459、462．790、74．434和675．500，P＜0．05）。与正常对照组相比，辐射组小鼠肺组织SOD活性下降，肺脏指数升高，细胞凋亡率增加，肺组织Bcl-2蛋白表达降低，Bax蛋白表达升高（P均＜0．05）。与辐射组比较，丙谷二肽组肺组织SOD活性显著升高，细胞凋亡率降低（P均＜0．05），细胞增殖率升高（t＝5．294，P＜0．05），Bcl-2/Bax比值显著提高（t＝218．920，P＜0．05）。结论：丙谷二肽对60Co γ射线辐射损伤小鼠肺组织具有一定的防护作用，这种保护作用可能与调控Bcl-2、Bax表达有关。
목적：탐토N（2）-L-병안선-L-곡안선알（병곡이태）대γ사선치소서폐조직손상적방호작용급가능적작용궤제。방법：장27지웅성곤명소서수궤분위정상대조조、복사조화병곡이태조，매조9지。병곡이태조안조1000 mg/kg적제량관위급약，1차/d，련속7 d。연후복사조、병곡이태조균급여4．0 Gy 60 Coγ사선조사3 d，정상대조조불작처리。조사후측정소서폐장지수、폐조직초양화물기화매（ SOD）활성，MTT법측정폐조직세포증식솔， AnnexinV화PI쌍염법측정세포조망솔，Western blot법검측폐조직Bcl-2화Bax단백적표체。결과：3조소서폐장지수、폐조직SOD활성、세포조망솔、폐조직Bcl-2화Bax단백표체수평차이균유통계학의의（ F＝6．352、51．459、462．790、74．434화675．500，P＜0．05）。여정상대조조상비，복사조소서폐조직SOD활성하강，폐장지수승고，세포조망솔증가，폐조직Bcl-2단백표체강저，Bax단백표체승고（P균＜0．05）。여복사조비교，병곡이태조폐조직SOD활성현저승고，세포조망솔강저（P균＜0．05），세포증식솔승고（t＝5．294，P＜0．05），Bcl-2/Bax비치현저제고（t＝218．920，P＜0．05）。결론：병곡이태대60Co γ사선복사손상소서폐조직구유일정적방호작용，저충보호작용가능여조공Bcl-2、Bax표체유관。
To investigate the possible mechanism involved in the protective effect of L-alanyl-L-glutamine ( Ala-Gln) against irradiation-injured lung tissue of mice .Methods:A total of 27 male Kunming mice were randomly assigned to control group , irradiated group and Ala-Gln-treated group ,9 in each group .Mice in the Ala-Gln-treated group were adminis-trated with 1 000 mg/kg Ala-Gln by gavage for 7 d.Then,the mice in irradiated group and Ala-Gln-treated group were irra-diated with 4.0 Gy 60Coγ-ray for 3 d.After irradiation, lung to body weight and superoxide dismutase (SOD) activity in lung tissue were detected;the proliferation and apoptosis were detected by MTT method and AnnexinV and PI staining ,re-spectively;the expressions of Bcl-2 and Bax proteins in lung tissue were detected by Western blot .Results: There were significant differences in lung to body weight ,SOD activity in lung tissue , apoptosis rate , and the expression levels of Bcl-2 and Bax proteins among the 3 groups(F=6.352,51.459,462.790,74.434 and 675.500,P<0.05).Compared with those of the control group , lung to body weight was higher , SOD activity was lower , apoptosis rate was higher , the Bcl-2 expression was lower and that of Bax protein was higher in irradiated group (P<0.05).Compared with those of the irradia-ted group, SOD activity in Ala-Gln-treated group increased, apoptosis rate decreased, and the proliferation rate and Bcl-2/Bax ratio were higher(t=5.294 and 218.920,P<0.05).Conclusion: Ala-Gln has radio-protective properties to lung in mice via modulating the expression levels of Bcl-2/Bax.