郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
4期
524-526
,共3页
乳癌%配对盒家族基因1%MDA-MB-231 细胞
乳癌%配對盒傢族基因1%MDA-MB-231 細胞
유암%배대합가족기인1%MDA-MB-231 세포
breast cancer%paired boxed gene 1%MDA-MB-231 cell
目的：研究Pax1在乳癌组织中的表达及其对乳癌MDA-MB-231细胞生物学行为的影响。方法：采用Western blot检测200乳腺原发癌和癌旁组织中Pax1蛋白的表达。采用化学合成针对Pax1基因的两个小干扰RNA （ siRNA-Pax1-1和siRNA-Pax1-2）下调该基因的表达，应用MTT和Transwell实验检测其对MDA-MB-231细胞生物学行为的影响。结果：Pax1在乳腺原发癌组织中的表达低于相应的癌旁组织，沉默Pax1表达可促进乳癌细胞增殖、迁移和侵袭（ F＝52．413和72．741，P＜0．001）。结论：Pax1有望成为乳癌早期诊断和预测预后的生物分子标志物。
目的：研究Pax1在乳癌組織中的錶達及其對乳癌MDA-MB-231細胞生物學行為的影響。方法：採用Western blot檢測200乳腺原髮癌和癌徬組織中Pax1蛋白的錶達。採用化學閤成針對Pax1基因的兩箇小榦擾RNA （ siRNA-Pax1-1和siRNA-Pax1-2）下調該基因的錶達，應用MTT和Transwell實驗檢測其對MDA-MB-231細胞生物學行為的影響。結果：Pax1在乳腺原髮癌組織中的錶達低于相應的癌徬組織，沉默Pax1錶達可促進乳癌細胞增殖、遷移和侵襲（ F＝52．413和72．741，P＜0．001）。結論：Pax1有望成為乳癌早期診斷和預測預後的生物分子標誌物。
목적：연구Pax1재유암조직중적표체급기대유암MDA-MB-231세포생물학행위적영향。방법：채용Western blot검측200유선원발암화암방조직중Pax1단백적표체。채용화학합성침대Pax1기인적량개소간우RNA （ siRNA-Pax1-1화siRNA-Pax1-2）하조해기인적표체，응용MTT화Transwell실험검측기대MDA-MB-231세포생물학행위적영향。결과：Pax1재유선원발암조직중적표체저우상응적암방조직，침묵Pax1표체가촉진유암세포증식、천이화침습（ F＝52．413화72．741，P＜0．001）。결론：Pax1유망성위유암조기진단화예측예후적생물분자표지물。
To investigate the expression of paired boxed gene 1 ( Pax1) gene in breast cancer tissue and to ex-plore the influence on proliferation status , migration ability and metastatic ability of breast cancer cells MDA-MB-231 . Methods:Western blot were used to examine the expression of Pax 1 in samps, including 200 normal tissues and 200 pri-mary tumors.MTT and transwell were used to analyze the ability of proliferation , migration, and invasion in Pax1 siRNA-transfected MDA-MB-231 cells.Results: The expression of Pax1 was lower in breast cancer tissues than other tissues . Furthermore, knockdown of Pax1 expression resulted in increased proliferation and invasion in MDA-MB-231 cells in vitro (F=52.413 and 72.741,P<0.001).Conclusion:The expression of Pax1 gene shows and obvious downgraded in breast cancer tissue.Low expression of Pax1 gene can increase apeptosis and proliteration of MDA-MB-231 cells, which may pro-vide a strategy for blocking breast cancer and progression .
