CAJ | 학술논문

目的：探讨羧甲基壳聚糖温敏凝胶对NIH3 T3细胞增殖、骨化分化的影响。方法：采用MTT法和ALP法检测20、100、150、200、500 g/L的羧甲基壳聚糖温敏凝胶浸提液对体外培养NIH3T3细胞24、48和72 h后细胞增殖、骨化分化的影响。结果：2种测定方法均显示培养24、48和72 h后各实验组细胞的OD值高于对照组（增殖：F组别＝113．961，F时间＝429．535，F交互＝7．304，P均＜0．001；骨化分化：F组别＝57．043，F时间＝3177．618，F交互＝12．827，P均＜0．001），其中150 g/L羧甲基壳聚糖温敏凝胶浸提液对细胞的促进增殖和骨化分化效果最佳。结论：羧甲基壳聚糖温敏凝胶对NIH3T3细胞的增殖及骨化分化均具有一定的促进作用。
목적：탐토최갑기각취당온민응효대NIH3 T3세포증식、골화분화적영향。방법：채용MTT법화ALP법검측20、100、150、200、500 g/L적최갑기각취당온민응효침제액대체외배양NIH3T3세포24、48화72 h후세포증식、골화분화적영향。결과：2충측정방법균현시배양24、48화72 h후각실험조세포적OD치고우대조조（증식：F조별＝113．961，F시간＝429．535，F교호＝7．304，P균＜0．001；골화분화：F조별＝57．043，F시간＝3177．618，F교호＝12．827，P균＜0．001），기중150 g/L최갑기각취당온민응효침제액대세포적촉진증식화골화분화효과최가。결론：최갑기각취당온민응효대NIH3T3세포적증식급골화분화균구유일정적촉진작용。
To explore the effects of carboxymethyl chitosan thermosensitive hydrogel on NIH 3T3 cell prolifera-tion and ossification differentiation.Methods:MTT method and ALP method were used to test the effects of 20, 100, 150, 200, 500 g/L carboxymethyl chitosan thermosensitive hydrogel leaching liquors on NIH 3T3 cell proliferation and ossification differentiation which was cultured in vivo for 24, 48 and 72 h.Results: Both methods showed that OD values of various experimental groups cultured for 24, 48 and 72 h were higher than that of the control group (proliferation:Fgroup =113.961, Ftime =429.535, Finteraction =7.304, P<0.001;ossification differentiation:Fgroup =57.043, Ftime =3 177.618, Finteraction =12.827,P<0.001);150 g/L carboxymethyl chitosan thermosensitive hydrogel leaching liquor had the best cell prolifera -tion and ossification differentiation effects .Conclusion: Carboxymethyl chitosan thermosensitive hydrogel can promote NIH3T3 cell proliferation and ossification differentiation .