CAJ | 학술논문

目的：探讨除草剂2，4-D丁酯对金鱼血清过氧化物酶(POD)和酯酶(EST)同酶的影响。方法：根据半数致死浓度，设置0.5、1.0、1.5、2.0 mL/L 4个浓度组及0.9%生理盐水对照组，每个浓度组按0.1 mL/g腹腔注射金鱼，染毒24 h，断尾取血，离心，取上清液，聚丙烯酰胺方法检测金鱼血清POD和EST同工酶活性，实验数据采用ANOVA分析。结果：与对照组相比，2，4-D丁酯在0.5、1.5和2.0 mL/L浓度时可抑制金鱼血清POD、EST同工酶活性(P<0.05)，在1.0 mL/L浓度时可使金鱼血清EST同工酶活性增强(P<0.05)。2，4-D丁酯对EST同工酶迁移率的影响大于POD。结论：2，4-D丁酯对金鱼血清POD和EST同工酶活性均有影响，2，4-D丁酯可改变EST同工酶的迁移率。
목적：탐토제초제2，4-D정지대금어혈청과양화물매(POD)화지매(EST)동매적영향。방법：근거반수치사농도，설치0.5、1.0、1.5、2.0 mL/L 4개농도조급0.9%생리염수대조조，매개농도조안0.1 mL/g복강주사금어，염독24 h，단미취혈，리심，취상청액，취병희선알방법검측금어혈청POD화EST동공매활성，실험수거채용ANOVA분석。결과：여대조조상비，2，4-D정지재0.5、1.5화2.0 mL/L농도시가억제금어혈청POD、EST동공매활성(P<0.05)，재1.0 mL/L농도시가사금어혈청EST동공매활성증강(P<0.05)。2，4-D정지대EST동공매천이솔적영향대우POD。결론：2，4-D정지대금어혈청POD화EST동공매활성균유영향，2，4-D정지가개변EST동공매적천이솔。
To explore the effect of herbicide 2, 4-D butyl ester on the expressions of peroxidase (POD) and esterase (EST) in Carassius auratus serum. METHODS:0.5,1.0,1.5,2.0 mL/L concentrations of 2,4-D butyl ester (according to its 24 h LC50) and 0.9%saline control group were prepared. Carassius auratus received intraperitoneal injection of 0.1 mL/g dose of each concentration. Tail blood of Carassius auratus and the supernatant of centrifugation were collected after 24 h. SDS-polyacrylamide gel electrophoresis was used to test the toxicity on POD and EST. The experimental data were analysed with ANOVA. RESULTS:POD and EST enzyme activities were inhibited at low concentrations(0.5 mL/L),induced at medium concentrations(1.0 mL/L) and decreased at high concentrations(1.5,2.0 mL/L). Compared with the control group. the effect of 2,4-d butyl ester on migration rate of EST isoenzyme was greater than POD. CONCLUSION:2,4-D butyl ester affected the activities of POD and EST in Carassius auratus and 2,4-d butyl ester changed the EST isoenzyme mobility.