CAJ | 학술논문

目的：研究甘肃地区汉族妇女XRCC1 rs25487、CCNH rs2234942基因多态性与乳腺癌及乳腺良性肿瘤发病的相关性。方法：选取经病理组织学确诊的乳腺癌、乳腺良性肿瘤各101例，匹配相同数量健康人作对照。使用聚合酶链式反应-限制性片段长度多态分析技术(PCR-RFLP)对XRCC1、CCNH进行基因型分析，通过Logistic回归分析不同基因型和临床病理特征与乳腺癌发病2的风险性关系，通过检验比较两种基因位点不同基因型的初潮年龄、发病年龄与乳腺癌和乳腺良性肿瘤的相关性。结果：Logistic回归分析发现XRCC1 rs25487位点GG基因型携带者的妇女罹患乳腺癌的危险性增加(P=0.001，OR=6.39，95%CI：2.18～+-18.65)；临床病理免疫组化分析显示，XRCC1基因rs25487位点携带AA/AG基因型者，在PR与PR乳腺癌组织间的分布差异有显著+-2性(P=0.04，OR=0.29)；携带AG/GG基因型者，在Her-2与Her-2乳腺癌组织间的分布差异有显著性(P=0.008，OR=0.45)。检验显示，乳腺癌和乳腺良性肿瘤患者XRCC1 rs25487位点GG/AG基因型携带者的初潮年龄差异有显著性(P=0.001、0.043)；乳腺癌和乳腺良性肿瘤患者CCNH rs2234942位点GG基因型携带者的初潮年龄差异有显著性(P=0.049)；乳腺癌和乳腺良性肿瘤患者XRCC1 rs25487和CCNH rs2234942位点GG基因型携带者，发病年龄差异有显著性(P=0.019、0.048)。结论：XRCC1 rs25487 GG基因型将会+增加乳腺癌的发病风险，XRCC1 rs25487位点携带AA/AG基因型者，在PR时乳腺癌的发病风险下降；携带AG/GG基因型者，在+Her-2时可能降低乳腺癌的发病风险。χχ
목적：연구감숙지구한족부녀XRCC1 rs25487、CCNH rs2234942기인다태성여유선암급유선량성종류발병적상관성。방법：선취경병리조직학학진적유선암、유선량성종류각101례，필배상동수량건강인작대조。사용취합매련식반응-한제성편단장도다태분석기술(PCR-RFLP)대XRCC1、CCNH진행기인형분석，통과Logistic회귀분석불동기인형화림상병리특정여유선암발병2적풍험성관계，통과검험비교량충기인위점불동기인형적초조년령、발병년령여유선암화유선량성종류적상관성。결과：Logistic회귀분석발현XRCC1 rs25487위점GG기인형휴대자적부녀리환유선암적위험성증가(P=0.001，OR=6.39，95%CI：2.18～+-18.65)；림상병리면역조화분석현시，XRCC1기인rs25487위점휴대AA/AG기인형자，재PR여PR유선암조직간적분포차이유현저+-2성(P=0.04，OR=0.29)；휴대AG/GG기인형자，재Her-2여Her-2유선암조직간적분포차이유현저성(P=0.008，OR=0.45)。검험현시，유선암화유선량성종류환자XRCC1 rs25487위점GG/AG기인형휴대자적초조년령차이유현저성(P=0.001、0.043)；유선암화유선량성종류환자CCNH rs2234942위점GG기인형휴대자적초조년령차이유현저성(P=0.049)；유선암화유선량성종류환자XRCC1 rs25487화CCNH rs2234942위점GG기인형휴대자，발병년령차이유현저성(P=0.019、0.048)。결론：XRCC1 rs25487 GG기인형장회+증가유선암적발병풍험，XRCC1 rs25487위점휴대AA/AG기인형자，재PR시유선암적발병풍험하강；휴대AG/GG기인형자，재+Her-2시가능강저유선암적발병풍험。χχ
To study the association between XRCC1 rs25487 and CCNH rs2234942 polymorphism with the susceptibility to breast cancer and benign breast tumor in Han women in Gansu area. METHODS:Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used for the polymorphism of XRCC1,CCNH in 101 cases of breast cancer,101 benign breast tumors and 101 disease-free controls collected in Gansu. Logistic regression analysis was used for comparing the genotypes or clinical pathological characteristics with the risk of breast cancer. Chi-square test was used to compare menarche age and disease onset age with the risk of breast cancer or benign tumor. RESULTS:Logistic regression analysis showed that XRCC1 rs25487,GG genotype increased the risk of breast cancer (P=0.001,OR=6.39,95%CI:2.18-18.65). Regarding the clinicopathological immunohistochemical characteristics,the distribution of AA/AG genotype was significantly different+ -between PR and PR at XRCC1 rs25487(P=0.04,OR=0.29),while the distribution of AG/GG genotype was significantly+ -different in Her-2 cases and Her-2 (P=0.008,OR=0.45). Chi-square test found that the menarche age of GG/AG genotype in XRCC1 rs25487 and GG genotype in CCNH rs2234942 was significantly different in breast cancer and benign tumor (P=0.001,0.043,0.049). The disease onset age of GG genotype in both XRCC1 rs25487 and CCNH rs2234942 was significantly different in breast cancer and benign tumor(P=0.019,0.048). CONCLUSION:GG genotype in+XRCC1 rs25487 increased the risk of breast cancer. AA/AG genotype in XRCC1 rs25487 with PR and AG/GG genotype in+CCNH rs2234942 with Her-2 reduced the risk of breast cancer.