癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2014年
4期
288-291,297
,共5页
食管黏膜病变%MMP-2%E-cadherin%免疫组化
食管黏膜病變%MMP-2%E-cadherin%免疫組化
식관점막병변%MMP-2%E-cadherin%면역조화
esophageal mucosal lesion%MMP-2%E-cadherin%immunohistochemistry
目的：探讨MMP-2和E-cadherin蛋白在食管黏膜病变中的表达及其与食管癌的发生及转移的关系。方法：采用免疫组织化学方法对45例食管癌组织、21例不典型增生组织、24例正常组织中MMP-2和E-cadherin蛋白的表达进行检测，并分析MMP-2和E-cadherin表达与食管癌发生发展过程中各项病理指标的关系。结果：MMP-2蛋白在癌组织中的表达率为40.00%(18/45)，在不典型增生组织中的表达率为38.10%(8/21)，在正常组织中表达率为4.17%(1/24)，三者间差异具有统计学意义(P<0.05)；有淋巴结转移组的MMP-2蛋白阳性表达率(55.56%，15/27)明显高于无淋巴结转移组(16.67%，3/18)；有纤维膜浸润组MMP-2蛋白的阳性表达率(48.57%，17/35)明显高于无纤维膜浸润组(10.00%，1/10)。E-cadherin蛋白在正常的食管黏膜上皮组织阳性表达为100%(24/24)，在不典型增生组织中的阳性表达率为71.43%(15/21)，在癌组织中的阳性表达率为28.89%(13/45)，较前两组明显降低(P<0.05)。E-cadherin与MMP-2蛋白的表达呈负相关(=4.615，r=-0.793，P<0.05)。结论：MMP-2蛋白的高表达和E-cadherin蛋白的低表达可能与食管癌的发生和转移有关，在食管癌的侵袭、转移过程中MMP-2与E-cadherin有拮抗作用。
目的：探討MMP-2和E-cadherin蛋白在食管黏膜病變中的錶達及其與食管癌的髮生及轉移的關繫。方法：採用免疫組織化學方法對45例食管癌組織、21例不典型增生組織、24例正常組織中MMP-2和E-cadherin蛋白的錶達進行檢測，併分析MMP-2和E-cadherin錶達與食管癌髮生髮展過程中各項病理指標的關繫。結果：MMP-2蛋白在癌組織中的錶達率為40.00%(18/45)，在不典型增生組織中的錶達率為38.10%(8/21)，在正常組織中錶達率為4.17%(1/24)，三者間差異具有統計學意義(P<0.05)；有淋巴結轉移組的MMP-2蛋白暘性錶達率(55.56%，15/27)明顯高于無淋巴結轉移組(16.67%，3/18)；有纖維膜浸潤組MMP-2蛋白的暘性錶達率(48.57%，17/35)明顯高于無纖維膜浸潤組(10.00%，1/10)。E-cadherin蛋白在正常的食管黏膜上皮組織暘性錶達為100%(24/24)，在不典型增生組織中的暘性錶達率為71.43%(15/21)，在癌組織中的暘性錶達率為28.89%(13/45)，較前兩組明顯降低(P<0.05)。E-cadherin與MMP-2蛋白的錶達呈負相關(=4.615，r=-0.793，P<0.05)。結論：MMP-2蛋白的高錶達和E-cadherin蛋白的低錶達可能與食管癌的髮生和轉移有關，在食管癌的侵襲、轉移過程中MMP-2與E-cadherin有拮抗作用。
목적：탐토MMP-2화E-cadherin단백재식관점막병변중적표체급기여식관암적발생급전이적관계。방법：채용면역조직화학방법대45례식관암조직、21례불전형증생조직、24례정상조직중MMP-2화E-cadherin단백적표체진행검측，병분석MMP-2화E-cadherin표체여식관암발생발전과정중각항병리지표적관계。결과：MMP-2단백재암조직중적표체솔위40.00%(18/45)，재불전형증생조직중적표체솔위38.10%(8/21)，재정상조직중표체솔위4.17%(1/24)，삼자간차이구유통계학의의(P<0.05)；유림파결전이조적MMP-2단백양성표체솔(55.56%，15/27)명현고우무림파결전이조(16.67%，3/18)；유섬유막침윤조MMP-2단백적양성표체솔(48.57%，17/35)명현고우무섬유막침윤조(10.00%，1/10)。E-cadherin단백재정상적식관점막상피조직양성표체위100%(24/24)，재불전형증생조직중적양성표체솔위71.43%(15/21)，재암조직중적양성표체솔위28.89%(13/45)，교전량조명현강저(P<0.05)。E-cadherin여MMP-2단백적표체정부상관(=4.615，r=-0.793，P<0.05)。결론：MMP-2단백적고표체화E-cadherin단백적저표체가능여식관암적발생화전이유관，재식관암적침습、전이과정중MMP-2여E-cadherin유길항작용。
To explore the relationship between the protein expressions of MMP-2 and E-cadherin and the development and metastasis of esophageal carcinoma. METHODS:The expressions of E-cadherin protein and MMP-2 protein were examined by immunohistochemistry (IHC) in 45 cases of esophageal carcinoma,21 cases of atypical hyperplasia and 24 cases of normal esophageal mucosa tissues. RESULTS:The positve rates of MMP-2 protein were 40.00%(18/45) in esophageal carcinoma,38.1%(8/21) in esophageal atypical hyperplasia and 4.17%(1/24) in normal tissues. There were significant differences among three groups (P<0.05). Additionally,the expression of MMP-2 protein had a correlation with lymph node metastasis (P<0.05). The positve rate of MMP-2 protein in esophageal cancer tissues with lymph node metastasis was 55.56% (15/27) , significantly higher than that in esophageal cancer tissues without lymph node metastasis (16.67%,3/18). The positve rate of MMP-2 protein in esophageal cancer tissues with fibrous membrane infiltration was higher than that in esophageal cancer tissues without fibrous membrane infiltration(48.57%,17/35;10.00%,1/10). The positive rate of E-cadherin protein in normal tissues (100%) was much higher than that in esophageal carcinoma (28.89%) and esophageal atypical hyperplasia (71.43%). According to these results,the expression of E-cadherin protein had a negative correlation with the expression of MMP-2 protein 2( =4.615,r=-0.793,P<0.05). CONCLUSION:There were significant relationships between the high expression of MMP-2 protein and low expression of E-cadherin protein with the development and metastasis of esophageal carcinoma.Furthermore,the expression of E-cadherin protein had a negative correlation with the expression of MMP-2 protein in the invasion and metastasis of esophageal carcinoma.
