癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2014年
4期
247-253
,共7页
zeste基因增强子人类同源物2%胚胎%发育%表达模式
zeste基因增彊子人類同源物2%胚胎%髮育%錶達模式
zeste기인증강자인류동원물2%배태%발육%표체모식
enhancer zeste homologue 2%fetus%development%expression pattern
目的：检测果蝇zeste基因增强子人类同源物2(EZH2)蛋白在人胎儿发育过程中的表达模式，为了解EZH2在胎儿发育过程中可能的作用，提供新的、多器官的信息。方法：收集不同胎龄流产或死产胎儿33例及1例新生儿标本。选取食管、胃、小肠、结肠、肝、胰、腮腺、甲状腺、肾上腺、肺、气管、心脏、大血管(主动脉或肺动脉)、肾、膀胱、子宫、卵巢、睾丸、脾、胸腺、大脑等器官组织，经HE染色辨别形态学结构并构建组织芯片后，进行EZH2免疫组化染色检测。结果：在所有检测的上述胎儿器官中EZH2蛋白均有表达，但在不同的组织器官和不同的胎龄其表达水平不同。EZH2蛋白阳性染色主要定位于细胞核，在部分细胞同时也有细胞质阳性染色。结论：EZH2在人类胎儿期的各器官组织均有表达，表达模式有胎儿发育时间的差异性和高度的组织特异性。
目的：檢測果蠅zeste基因增彊子人類同源物2(EZH2)蛋白在人胎兒髮育過程中的錶達模式，為瞭解EZH2在胎兒髮育過程中可能的作用，提供新的、多器官的信息。方法：收集不同胎齡流產或死產胎兒33例及1例新生兒標本。選取食管、胃、小腸、結腸、肝、胰、腮腺、甲狀腺、腎上腺、肺、氣管、心髒、大血管(主動脈或肺動脈)、腎、膀胱、子宮、卵巢、睪汍、脾、胸腺、大腦等器官組織，經HE染色辨彆形態學結構併構建組織芯片後，進行EZH2免疫組化染色檢測。結果：在所有檢測的上述胎兒器官中EZH2蛋白均有錶達，但在不同的組織器官和不同的胎齡其錶達水平不同。EZH2蛋白暘性染色主要定位于細胞覈，在部分細胞同時也有細胞質暘性染色。結論：EZH2在人類胎兒期的各器官組織均有錶達，錶達模式有胎兒髮育時間的差異性和高度的組織特異性。
목적：검측과승zeste기인증강자인류동원물2(EZH2)단백재인태인발육과정중적표체모식，위료해EZH2재태인발육과정중가능적작용，제공신적、다기관적신식。방법：수집불동태령유산혹사산태인33례급1례신생인표본。선취식관、위、소장、결장、간、이、시선、갑상선、신상선、폐、기관、심장、대혈관(주동맥혹폐동맥)、신、방광、자궁、란소、고환、비、흉선、대뇌등기관조직，경HE염색변별형태학결구병구건조직심편후，진행EZH2면역조화염색검측。결과：재소유검측적상술태인기관중EZH2단백균유표체，단재불동적조직기관화불동적태령기표체수평불동。EZH2단백양성염색주요정위우세포핵，재부분세포동시야유세포질양성염색。결론：EZH2재인류태인기적각기관조직균유표체，표체모식유태인발육시간적차이성화고도적조직특이성。
To determine the expression pattern of EZH2 protein in the process of human fetal development and provide new and multi-organ information for understanding the possible roles of EZH2 during the whole fetal development. METHODS:33 human fetuses at 25 different gestational weeks and 1 neonatal sample were obtained through abortion or stillbirth. The organ or tissue including esophagus,stomach,small intestine,colon,liver, pancreas,parotid gland,thyroid,adrenal gland,lung,trachea,heart,large artery,kidney,bladder,uterus, ovary,testis,spleen,thymus,cerebrum were fixed,embedded,sectioned,then stained with hematoxylin and eosin to identify their morphological structures. Tissue microarrays were constructed and followed by immunohistochemical staining of EZH2. RESULTS:EZH2 was expressed in fetal organs investigated and exhibited different expression levels in different tissues and at various gestational ages. The positive staining of EZH2 protein was mainly located in the nucleus,as well as in the cytoplasm in some cells. CONCLUSION:EZH2 protein was generally expressed in human fetal organs and tissues and its expression exhibited tissue specificity and dependence on the stages of fetal development.
