CAJ | 학술논문

目的：探索一种提高总RNA提取浓度的方法。方法：用 Trizol方法分别提取乳腺癌细胞和心肌组织中的总RN A。在提取过程中，分别把加入异丙醇后的混合液8等份，其中4个样品管分别在室温、4℃、-20℃、-80℃下放置10 min ，另外4个样品管在-80℃冰箱中分别放置0．5、1、2、4 h；然后检测提取到的总RNA质量浓度、纯度及完整性。结果：均放置10 min时，提取的总RNA质量浓度随放置温度的降低而增加（P≤0．01）；放置于-80℃时，随放置时间的增加，提取的总RNA浓度增加（P≤0．01）。两种条件下提取的总RNA的纯度均无显著性差异（P＞0．05），其完整性也无明显影响。结论：不同的放置时间和温度会影响RN A提取的质量浓度，但对RN A纯度和完整性无明显影响。
목적：탐색일충제고총RNA제취농도적방법。방법：용 Trizol방법분별제취유선암세포화심기조직중적총RN A。재제취과정중，분별파가입이병순후적혼합액8등빈，기중4개양품관분별재실온、4℃、-20℃、-80℃하방치10 min ，령외4개양품관재-80℃빙상중분별방치0．5、1、2、4 h；연후검측제취도적총RNA질량농도、순도급완정성。결과：균방치10 min시，제취적총RNA질량농도수방치온도적강저이증가（P≤0．01）；방치우-80℃시，수방치시간적증가，제취적총RNA농도증가（P≤0．01）。량충조건하제취적총RNA적순도균무현저성차이（P＞0．05），기완정성야무명현영향。결론：불동적방치시간화온도회영향RN A제취적질량농도，단대RN A순도화완정성무명현영향。
Objective:To investigate a method of increasing concentration for total RNA isolation Methods :We isolated the total RNA from rats heart tissue and cell by the Trizol .The mixture of isopropanol is divided into eight equal parts during the process of total RNA isolation .Four simples are placed at room temperature ,4℃ ,-20 ℃ ,-80 ℃ for 10 min , respectively ;others are placed at -80 ℃ for 0 .5 h ,1 h ,2 h ,4 h , respectively .The concentration ,the purity and the integrity of each sample were detected .Results:The concentration of total RNA increases with the decreasing temperature when they are placed for 10min(P≤0.01) and with the increasing storage time at -80 ℃(P≤0 .01) .There is no significant difference about the purity and integrity of total RNA isolated from rats heart tissue and cell .Conclusion:Placing at different temperature or lasting for different time could affect the concentration ,but not the purity and integrity of total RN A .