CAJ | 학술논문

过氧化物酶体膜蛋白PMP是ABC转运蛋白ABCD亚家族蛋白之一，存在于过氧化物酶体膜上。研究表明，拟南芥中的ABCD亚家族ABC转运蛋白AtABCD1主要通过参与部分物质运输进入过氧化物酶体，其中包括茉莉酸合成的前体OPDA。本研究通过RACE技术克隆了一个ABCD亚家族ABC转运蛋白基因HbPMP1，并进行表达分析。结果表明： HbPMP1全长4011 bp，编码1337个氨基酸残基，其表达产物与拟南芥AtABCD1有较高的相似性(氨基酸一致性为78%)；该基因在伤害及外源茉莉酸诱导下呈上调表达，推测其可能参与内源茉莉酸合成相关物质时的运输。
과양화물매체막단백PMP시ABC전운단백ABCD아가족단백지일，존재우과양화물매체막상。연구표명，의남개중적ABCD아가족ABC전운단백AtABCD1주요통과삼여부분물질운수진입과양화물매체，기중포괄말리산합성적전체OPDA。본연구통과RACE기술극륭료일개ABCD아가족ABC전운단백기인HbPMP1，병진행표체분석。결과표명： HbPMP1전장4011 bp，편마1337개안기산잔기，기표체산물여의남개AtABCD1유교고적상사성(안기산일치성위78%)；해기인재상해급외원말리산유도하정상조표체，추측기가능삼여내원말리산합성상관물질시적운수。
Peroxisomal membrane protein PMP is one of the ABC transporter ABCD subfamily protein,and exists in the peroxisomal membrane. Research shows that AtABCD1, a Arabidopsis ABCD subfamily protein, mainly involved material input in peroxisome such as jasmonic acid synthesis precursor OPDA. In this study, a ABCD subfamily ABC transporter gene HbPMP1 was cloned by RACE, which contains a ORF with 4 011 bp and encodes a polypeptide of 1 337 amino acids, HbPMP1 had higher similarity with Arabidopsis AtABCD1 (78% amino acid identity). Expression analysis indicated that HbPMP1 was up-regulated by the injury and exogenous jasmonic acid, suggesting that HbPMP1 may be involved in substance transport relating to endogenous jasmonic acid biosynthesis.