CAJ | 학술논문

将海洋多粘类芽孢杆菌L1-9菌株接种在日本囊对虾养殖池水体中，以不接L1-9菌株为对照组，定期检测水体中L1-9菌株、弧菌、芽孢杆菌及其他异养细菌数量以及氨氮、硝酸盐、亚硝酸盐和磷酸盐的质量浓度和日本囊对虾的成活率。试验结果表明，在日本囊对虾养殖池水体中，随着培养时间的延长，接种L1-9菌株池水中，芽孢杆菌、异养细菌和弧菌的数量以及氨氮、硝酸盐、亚硝酸盐含量显著低于对照组，磷酸盐的质量浓度显著高于对照组，其中对芽孢杆菌和氨氮的影响最为明显，培养第5 d处理组芽孢杆菌的菌落数为4．60×102 cfu/mL显著低于对照组的1．80×103 cfu/mL ；培养第7 d处理组的氨氮质量浓度为60．2μg/L ，显著低于对照组的76．7μg/L ，表明L1-9菌株在水产养殖中具有一定抑菌及水质净化作用，L1-9菌株对日本囊对虾成活率没有影响。
장해양다점류아포간균L1-9균주접충재일본낭대하양식지수체중，이불접L1-9균주위대조조，정기검측수체중L1-9균주、호균、아포간균급기타이양세균수량이급안담、초산염、아초산염화린산염적질량농도화일본낭대하적성활솔。시험결과표명，재일본낭대하양식지수체중，수착배양시간적연장，접충L1-9균주지수중，아포간균、이양세균화호균적수량이급안담、초산염、아초산염함량현저저우대조조，린산염적질량농도현저고우대조조，기중대아포간균화안담적영향최위명현，배양제5 d처리조아포간균적균락수위4．60×102 cfu/mL현저저우대조조적1．80×103 cfu/mL ；배양제7 d처리조적안담질량농도위60．2μg/L ，현저저우대조조적76．7μg/L ，표명L1-9균주재수산양식중구유일정억균급수질정화작용，L1-9균주대일본낭대하성활솔몰유영향。
Marine Paenibacillus polymyxa strain L1-9 was inoculated (without inoculation as control) in Japanese shrimp culture ponds to investigate the colonization ,bacteriostasis and purification water of the marine P .polymyxa strain L1-9 in the Japanese shrimp culture ponds .After that ,the number of L1-9 , V ibrio ,Bacillaceae and heterotrophic bacteria was detected regularly ,and the concentrations of ammonia nitrogen ,nitrate , nitrite , and phosphate were detected . During the culture , the number of V ibrio , Bacillaceae ,and heterotrophic bacteria and the levels of ammonia nitrogen ,nitrate ,and nitrite were found to be significantly lower in the Japanese shrimp culture ponds inoculated with L 1-9 strain than those in the control . However , there was significantly higher concentration of phosphate in the Japanese shrimp culture ponds inoculated with L1-9 strain than that in the control pond , especially the number of Bacillaceae and the ammonia nitrogen level , the Bacillaceae reaching 4 .60 × 102 cfu/mL on the 5 th d , significantly less than that in the control (1 .80 × 103 cfu/mL) on the 7 th d .The concentration of ammonia nitrogen was found to be 60 .2 μg/L ,less than that in the control (76 .7 μg/L) .It is concluded that L1-9 strain has obviously bacteriostasis and purification water effect in aquaculture .