中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2013年
8期
777-779
,共3页
李震%郑豪%李国宾%智会%袁维堂
李震%鄭豪%李國賓%智會%袁維堂
리진%정호%리국빈%지회%원유당
慢传输型便秘%Cajal间质细胞%c-kit蛋白%动物模型,大鼠
慢傳輸型便祕%Cajal間質細胞%c-kit蛋白%動物模型,大鼠
만전수형편비%Cajal간질세포%c-kit단백%동물모형,대서
Slow transit constipation%Interstitial cells of Cajal%c-kit%Animal model,rat
目的 研究Cajal间质细胞(ICC)与慢传输型便秘(STC)的关系.方法 用复方地芬诺酯灌胃的方法建立大鼠慢传输便秘模型(STC组),Western blot法测定STC组与对照组大鼠升结肠和降结肠组织ICC的特异性标志物c-kit变化情况,利用其与对应的内参β-actin灰度值的比值作为各组c-kit蛋白相对含量.结果 STC组大鼠日均粪便量为(1.3±0.7) g/100 g,比对照组的(1.6±0.9) g/100 g明显减少,差异有统计学意义(t=10.798,P＜0.05).STC组首粒黑粪排出时间为(461.6±150.8) min,较对照组大鼠的(351.3±119.9) min显著延长(t=2.291,P＜0.05).STC组与对照组升结肠c-kit灰度比平均值分别为0.277±0.077和0.576±0.081 (t=10.719,P＜0.05)；降结肠c-kit灰度比平均值分别为0.280±0.075和0.571 ±0.079(t=10.700,P＜0.05)；c-kit在STC组升结肠和降结肠的表达均下调,差异均有统计学意义.结论 ICC在升结肠和降结肠的减少可能对慢传输型便秘的发生与发展有一定作用.
目的 研究Cajal間質細胞(ICC)與慢傳輸型便祕(STC)的關繫.方法 用複方地芬諾酯灌胃的方法建立大鼠慢傳輸便祕模型(STC組),Western blot法測定STC組與對照組大鼠升結腸和降結腸組織ICC的特異性標誌物c-kit變化情況,利用其與對應的內參β-actin灰度值的比值作為各組c-kit蛋白相對含量.結果 STC組大鼠日均糞便量為(1.3±0.7) g/100 g,比對照組的(1.6±0.9) g/100 g明顯減少,差異有統計學意義(t=10.798,P＜0.05).STC組首粒黑糞排齣時間為(461.6±150.8) min,較對照組大鼠的(351.3±119.9) min顯著延長(t=2.291,P＜0.05).STC組與對照組升結腸c-kit灰度比平均值分彆為0.277±0.077和0.576±0.081 (t=10.719,P＜0.05)；降結腸c-kit灰度比平均值分彆為0.280±0.075和0.571 ±0.079(t=10.700,P＜0.05)；c-kit在STC組升結腸和降結腸的錶達均下調,差異均有統計學意義.結論 ICC在升結腸和降結腸的減少可能對慢傳輸型便祕的髮生與髮展有一定作用.
목적 연구Cajal간질세포(ICC)여만전수형편비(STC)적관계.방법 용복방지분낙지관위적방법건립대서만전수편비모형(STC조),Western blot법측정STC조여대조조대서승결장화강결장조직ICC적특이성표지물c-kit변화정황,이용기여대응적내삼β-actin회도치적비치작위각조c-kit단백상대함량.결과 STC조대서일균분편량위(1.3±0.7) g/100 g,비대조조적(1.6±0.9) g/100 g명현감소,차이유통계학의의(t=10.798,P＜0.05).STC조수립흑분배출시간위(461.6±150.8) min,교대조조대서적(351.3±119.9) min현저연장(t=2.291,P＜0.05).STC조여대조조승결장c-kit회도비평균치분별위0.277±0.077화0.576±0.081 (t=10.719,P＜0.05)；강결장c-kit회도비평균치분별위0.280±0.075화0.571 ±0.079(t=10.700,P＜0.05)；c-kit재STC조승결장화강결장적표체균하조,차이균유통계학의의.결론 ICC재승결장화강결장적감소가능대만전수형편비적발생여발전유일정작용.
Objective To investigate the association of expression of c-kit (marker of interstitial cells of Cajal,ICC) in colon with slow transit constipation (STC) in rats.Methods Slow transit constipation (STC) rat model was induced by intragastrical administration of compound diphenoxylate.Western blotting was used to measure the expression of c-kit in colon of STC rats (model group) and normal rats(control group).Gray scale ratio of c-kit to β-actin was used as the relative quantity of c-kit.Results Fecal quantity per day of STC group was (1.3±0.7) g/100 g,significantly lower than that in normal rats [(1.6±0.9) g/100 g,t=10.798,P＜0.05].In model rats,the time of discharge of the first black fecal was (461.6±150.8)min,significantly longer than that in normal rats[(351.3±119.9)min,t=2.291,P＜0.05].Western blotting revealed that the average values of gray scale ratio of c-kit in proximal colon were 0.277±0.077 and 0.576±0.081 (t=10.719,P＜0.05),in distal colon were 0.280±0.075 and 0.571±0.079 (t=10.700,P＜0.05) in model group and control group respectively.Conclusion Down-regulation of c-kit expression in proximal colon and distal colon is associated to the pathogenesis of slow transit constipation in rats.
