CAJ | 학술논문

该研究建立高效液相色谱法快速测定荞麦制品中可可碱、咖啡因含量分析方法，采用甲醇∶水∶氨水（70∶3∶1，V/V/V）为提取液超声提取荞麦制品中的可可碱、咖啡因，在Shimpack VP-ODS色谱柱（250 mm×4.6 mm，5μm）上，以甲醇∶0.02 mol/L乙酸铵溶液为流动相进行梯度洗脱，紫外检测器275 nm波长检测。结果：采用外标法定量，该方法在0.25~50.0μg/mL浓度范围内有良好的线性关系，加标回收率均在97%以上，相对标准偏差不大于3.0%，可可碱最低检出限1.4 mg/kg；咖啡因最低检出限1.0 mg/kg，对荞麦制品中可可粉添加量的最低识别浓度为0.01%。结论：该方法具有快速、准确、灵敏度高，满足实验室日常鉴别荞麦制品中是否添加可可粉的工作需求。
해연구건립고효액상색보법쾌속측정교맥제품중가가감、가배인함량분석방법，채용갑순∶수∶안수（70∶3∶1，V/V/V）위제취액초성제취교맥제품중적가가감、가배인，재Shimpack VP-ODS색보주（250 mm×4.6 mm，5μm）상，이갑순∶0.02 mol/L을산안용액위류동상진행제도세탈，자외검측기275 nm파장검측。결과：채용외표법정량，해방법재0.25~50.0μg/mL농도범위내유량호적선성관계，가표회수솔균재97%이상，상대표준편차불대우3.0%，가가감최저검출한1.4 mg/kg；가배인최저검출한1.0 mg/kg，대교맥제품중가가분첨가량적최저식별농도위0.01%。결론：해방법구유쾌속、준학、령민도고，만족실험실일상감별교맥제품중시부첨가가가분적공작수구。
a method was developed for the determination of theobromine and caffeine content of buckwheat products using high performance liquid chromatography. Method:the sample was ultrasonic extracted with methanol-water-ammonia water(70∶30∶1,V/V/V),and separated in Shimpack VP-ODS column(250 mm×4.6 mm,5μm)using methanol-0.02 mol/L ammonium acetate solution as mobile phase by gradient elution,and determined at 257nm by UV detector. Results:the calibration curve at the concentration range of 0.25~50.0μg ml-1 was good linear. The recoveries of theobromine and caffeine in buckwheat products were above 97%and the relative standard deviation was not more than 3%. The lowest detection limit for theobromine was 1.4 mg/kg-1 and for caffeine 1.0 mg/kg. The lowest concentration of recognition for cocoa powder in buckwheat products was 0.01%. Conclusions:This method has the advantage of speedy,exactness and high sensitivity,which meets the demand of distinguishing cocoa powder added or not in buckwheat products.