CAJ | 학술논문

该实验采用双水相萃取法提取米糠脂肪酶，通过确定米糠脂肪酶最佳提取条件，为日后大规模生产提供理论依据。用蒸馏水对米糠脂肪酶粗提取实验，通过单因素实验确定最佳提取工艺参数：料液比1∶15，提取温度40℃，提取pH 7.0，提取时间1 h。双水相萃取对粗酶液纯化实验，对影响双水相萃取各种因素进行探讨，并通过正交实验优化提取条件，于PEG浓度22.5%，硫酸铵浓度20%，pH 7.0条件下进行双水相萃取，脂肪酶纯化倍数最高。
해실험채용쌍수상췌취법제취미강지방매，통과학정미강지방매최가제취조건，위일후대규모생산제공이론의거。용증류수대미강지방매조제취실험，통과단인소실험학정최가제취공예삼수：료액비1∶15，제취온도40℃，제취pH 7.0，제취시간1 h。쌍수상췌취대조매액순화실험，대영향쌍수상췌취각충인소진행탐토，병통과정교실험우화제취조건，우PEG농도22.5%，류산안농도20%，pH 7.0조건하진행쌍수상췌취，지방매순화배수최고。
This experiment adopts the aqueous two phase extraction method to extract rice bran lipase, through determine the optimal extraction conditions of rice bran lipase,provide theoretical basis for the mass production in the future. With distilled water to the rice bran lipase crude extraction experiment, the best extraction technology was determined by the single factor experiment parameters:the ratio of material to water 1∶15,extraction temperature 40℃,the extraction of pH 7.0,extracting time of 1h. Aqueous two-phase extraction of crude enzyme liquid purification experiment,discusses the factors affecting the aqueous two-phase extraction,and through the orthogonal experiment to optimize the extraction conditions of PEG concentration 22.5%,20% ammonium sulfate concentration,aqueous two-phase extraction under the condition of pH 7.0,lipase purified the highest multiples.