CAJ | 학술논문

目的：探讨CD152启动子-1722T/C、-1661A/G以及外显子1+49A/G基因多态性与儿童特发性脑卒中发生的关系。方法51例特发性脑卒中患儿作为病例组，74名健康汉族儿童作为对照组，采用PCR-RFLP方法进行CD152基因型分析。结果对照组和病例组不同CD152基因型分布经χ2检验符合Hardy-Weinberg平衡定律。CD152-1722 T/C、-1661A/G不同基因型及等位基因频率在病例组与对照组之间的差异无统计学意义（P均>0.05）；病例组的CD152+49GG基因型及G等位基因频率较对照组明显升高，差异有统计学意义（χ2=7.053、6.351，P<0.05）。CD152+49A/G基因型频率与常见临床表型（如偏瘫、言语障碍、惊厥、意识改变以及发病前感染）之间无相关性（P均>0.05）结论 CD152+49A/G基因多态性可能与特发性卒中发生风险有关。
목적：탐토CD152계동자-1722T/C、-1661A/G이급외현자1+49A/G기인다태성여인동특발성뇌졸중발생적관계。방법51례특발성뇌졸중환인작위병례조，74명건강한족인동작위대조조，채용PCR-RFLP방법진행CD152기인형분석。결과대조조화병례조불동CD152기인형분포경χ2검험부합Hardy-Weinberg평형정률。CD152-1722 T/C、-1661A/G불동기인형급등위기인빈솔재병례조여대조조지간적차이무통계학의의（P균>0.05）；병례조적CD152+49GG기인형급G등위기인빈솔교대조조명현승고，차이유통계학의의（χ2=7.053、6.351，P<0.05）。CD152+49A/G기인형빈솔여상견림상표형（여편탄、언어장애、량궐、의식개변이급발병전감염）지간무상관성（P균>0.05）결론 CD152+49A/G기인다태성가능여특발성졸중발생풍험유관。
Objectives To explore polymorphisms of CD152 gene promoters (-1722T/C,-1661A/G) and exon1 (+49A/G) in children with idiopathic ischemic stroke and assess the association between these polymorphisms with the disease. Methods Us-ing the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, polymorphisms in the CD152 exon1 region (+49A/G) and promoter regions (-1722T/C,-1661A/G) were genotyped in 51 Han children with idiopathic ischemic stroke and 74 healthy Han children. Results The CD152 genotypes in control group and idiopathic ischemic stroke children were consistent to principle of Hardy-Weinberg Equilibrium (HWE) byχ2 test. The CD152+49GG genotypes and G allele frequency in the patients showed a signiifcant increase compared to the controls (χ2=7.053, 6.351, P<0.05). However, the CD152-1722T/C,-1661A/G genotypes and allele frequency showed no signiifcant difference between 2 groups (P>0.05). Clinical features (hemi-paresis, dysarthria, seizures, consciousness change, and preceding infections) did not show signiifcant correlation with genotypes and frequency of the CD152+49 polymorphisms in children with idiopathic ischemic stroke (P>0.05). Conclusions The CD152+49A/G polymorphisms may relate to idiopathic ischemic stroke.