CAJ | 학술논문

建立基于邻苯二甲醛（ o-phthalaldehyde ， OPA）柱前衍生的高效液相色谱法（ high performance liquid chromato-graphy， HPLC），测定了稻米中γ-氨基丁酸（γ-aminobutyric acid， GABA）的含量。结果显示：用三氯乙酸作为提取溶剂的效果最好；检测限为0．5 mg/L，提取回收率为79．1％～101．5％；巨胚糙米的GABA含量显著高于常规胚糙米的，发芽后巨胚糙米的GABA积累量也高于常规胚糙米的。
건립기우린분이갑철（ o-phthalaldehyde ， OPA）주전연생적고효액상색보법（ high performance liquid chromato-graphy， HPLC），측정료도미중γ-안기정산（γ-aminobutyric acid， GABA）적함량。결과현시：용삼록을산작위제취용제적효과최호；검측한위0．5 mg/L，제취회수솔위79．1％～101．5％；거배조미적GABA함량현저고우상규배조미적，발아후거배조미적GABA적루량야고우상규배조미적。
The high performance liquid chromatography ( HPLC) method based on pre-column derivatization with o -phthal-aldehyde (OPA) was established, and it was used to determine the content of γ-aminobutyric acid (GABA) in rice.The results indicated that trichloroacetic acid used as solvent had the best extract effect ;the detection limit of this method was 0.5 mg/L, and the recoveries were 79.1%~101.5%;the content of GABA in brown rice with giant embryo was significantly higher than that in brown rice with normal embryo;after germination, the accumulation of GABA content in brown rice with giant embryo was also high-er than that in brown rice with normal embryo .