CAJ | 학술논문

通过盆栽实验，研究了不同盐浓度胁迫下狼尾蕨及其体细胞耐盐突变体NY-7的5个生理指标，并应用模糊隶属函数法对其耐盐性进行了综合评价。结果显示：随着NaCl浓度的升高和胁迫时间的延长，NY-7的叶绿素和可溶性蛋白含量下降，脯氨酸含量、SOD活性均没有显著变化，MDA含量有所增加；而狼尾蕨的叶绿素含量显著降低，SOD活性、脯氨酸、可溶性蛋白和MDA含量均显著增加；NY-7的耐盐性高于狼尾蕨。
통과분재실험，연구료불동염농도협박하랑미궐급기체세포내염돌변체NY-7적5개생리지표，병응용모호대속함수법대기내염성진행료종합평개。결과현시：수착NaCl농도적승고화협박시간적연장，NY-7적협록소화가용성단백함량하강，포안산함량、SOD활성균몰유현저변화，MDA함량유소증가；이랑미궐적협록소함량현저강저，SOD활성、포안산、가용성단백화MDA함량균현저증가；NY-7적내염성고우랑미궐。
Using Davallia bullata and its salt-tolerant somatic cell mutant NY -7 as experimental materials , we studied their 5 physiological indexes under different salt concentrations stress through pot experiments , and comprehensively evaluated their salt tolerance by using fuzzy subordinate function method .The results showed that:when salt concentration increased and stress time ex-tended, the contents of both leaf chlorophyll and soluble protein showed the trend of declining in salt -tolerant mutant NY-7, while its proline content and SOD activity did not present significant changes , and its content of MDA increased slightly .In comparison with NY-7, the leaf chlorophyll content of Davallia bullata significant decreased , and its SOD activity and the contents of proline , soluble protein and MDA significantly increased .The comprehensive evaluation of five physiological indexes suggested that the salt tolerance of NY-7 was higher than that of Davallia bullata.