CAJ | 학술논문

以棕榈科植物的DNA为材料，通过对SSR反应体系中模板DNA、dNTPs、引物、Taq聚合酶和MgCl25个因素采用单因素试验法设定5个梯度，并比较不同的浓度、不同的用量对扩增效果的影响，建立最佳反应体系。研究最终确定了20μL PCR反应体系的最佳条件为：模板DNA 2μL，dNTPs 0．2μL，引物1．0μL，Taq聚合酶0．2μL，MgCl22．0μL。该优化体系稳定可靠，适合应用于棕榈科植物SSR分析。
이종려과식물적DNA위재료，통과대SSR반응체계중모판DNA、dNTPs、인물、Taq취합매화MgCl25개인소채용단인소시험법설정5개제도，병비교불동적농도、불동적용량대확증효과적영향，건립최가반응체계。연구최종학정료20μL PCR반응체계적최가조건위：모판DNA 2μL，dNTPs 0．2μL，인물1．0μL，Taq취합매0．2μL，MgCl22．0μL。해우화체계은정가고，괄합응용우종려과식물SSR분석。
To establish the optimal SSR-PCR reaction system for palm trees , different factors that mostly affected the amplifi-cation of SSR-PCR reaction, including DNA template, dNTPs, primers, TaqDNA and MgCl2, were investigated and optimized by setting up 5 gradients with single-fact test method in the experiment .Meanwhile , we also compared the influences of different con-centrations and different consumptions on the amplification .The results showed that the optimal SSR -PCR system was DNA tem-plate 2 μL, dNTPs 0.2 μL, primers 1.0 μL, TaqDNA 0.2 μL, and MgCl2 2.0 μL, which was more stable and suitable for the SSR analysis of palm plants .