CAJ | 학술논문

目的：观察藏药十八味欧曲膏对2，4-二硝基氯苯(DNCB)致湿疹模型大鼠脾脏与胸腺指数及血清INF-γ表达的影响。方法：外用不同浓度DNCB反复刺激脱毛区，建立大鼠湿疹模型，采用藏药十八味欧曲膏低(0.14 g/kg)、中(0.21 g/kg)、高(0.35 g/kg)剂量经皮给药治疗，以冰黄肤乐、凡士林为对照，10天后检测脾脏和胸腺指数，同时采用ELISA法检测大鼠血清中的INF-γ的含量。结果：与模型对照组比较，胸腺及脾脏指数冰黄肤乐阳性对照组、藏药十八味欧曲膏低剂量组、中剂量组、高剂量组均显著降低(P＜0.05)，其中藏药十八味欧曲膏高、中剂量组优于低剂量组(P＜0.05)，藏药十八味欧曲膏低剂量组与凡士林组无明显差异(P＞0.05)，凡士林组与模型对照组无明显差异(P＞0.05)；藏药十八味欧曲膏干预后INF-γ水平明显提高，与治疗前比较有显著性差异(P＜0.01)，其中高、中、低剂量组与模型对照组比较有显著差异(P＜0.05)，低剂量组与凡士林组比较无显著差异(P＞0.05)。结论：藏药十八味欧曲膏可调节T细胞亚群的失衡状况，具有免疫调节作用。
목적：관찰장약십팔미구곡고대2，4-이초기록분(DNCB)치습진모형대서비장여흉선지수급혈청INF-γ표체적영향。방법：외용불동농도DNCB반복자격탈모구，건립대서습진모형，채용장약십팔미구곡고저(0.14 g/kg)、중(0.21 g/kg)、고(0.35 g/kg)제량경피급약치료，이빙황부악、범사림위대조，10천후검측비장화흉선지수，동시채용ELISA법검측대서혈청중적INF-γ적함량。결과：여모형대조조비교，흉선급비장지수빙황부악양성대조조、장약십팔미구곡고저제량조、중제량조、고제량조균현저강저(P＜0.05)，기중장약십팔미구곡고고、중제량조우우저제량조(P＜0.05)，장약십팔미구곡고저제량조여범사림조무명현차이(P＞0.05)，범사림조여모형대조조무명현차이(P＞0.05)；장약십팔미구곡고간예후INF-γ수평명현제고，여치료전비교유현저성차이(P＜0.01)，기중고、중、저제량조여모형대조조비교유현저차이(P＜0.05)，저제량조여범사림조비교무현저차이(P＞0.05)。결론：장약십팔미구곡고가조절T세포아군적실형상황，구유면역조절작용。
Objective: To observe the effects of Tibetan drug ShiBaWei OuQu ointment on serum INF-γexpressions, spleen and thymus indexes of rat model with eczema induced by DNCB. Method: Rat model was established and treated with Tibetan drug ShiBaWei OuQu ointment in different dosages:low (0.14 g/kg), moderate (0.21 g/kg) and high (0.35 g/kg), compared with BingHuang FuLe and petroleum jelly, spleen and thymus indexes were tested in ten days, the contents of INF-γ were detected with ELISA method. Result: Compared with model control group, spleen and thymus indexes in positive control group of BingHuang FuLe, low, moderate and high dosages groups of Tibetan drug ShiBaWei OuQu ointment were decreased significantly(P<0.05), moderate and high dosages groups of Tibetan drug ShiBaWei OuQu ointment were superior to low dosage group of Tibetan drug ShiBaWei OuQu ointment (P<0.05), it had no significant difference between low dosage group of Tibetan drug ShiBaWei OuQu ointment and petroleum jelly group (P>0.05), also between petroleum jelly group and model control group (P>0.05); after intervened with Tibetan drug ShiBaWei OuQu ointment, the level of INF-γ was increased obviously, it had significant difference compared with the rats before treating (P<0.01), it had notable difference when low, moderate and high dosages groups of Tibetan drug ShiBaWei OuQu ointment were compared with model control group (P<0.05), it had insignificant difference between low dosage group of Tibetan drug ShiBaWei OuQu ointment and petroleum jelly group (P>0.05).Conclusion:Tibetan drug ShiBaWei OuQu ointment could regulate the imbalance of T lymphocyte subsets and it shows the function of immunoregulation.