CAJ | 학술논문

动物胃肠道中存在复杂多样的微生物，但只有少数微生物能够用传统方法进行纯培养。利用宏基因组学方法研究动物胃肠道微生物绕过纯培养技术，不仅可以发现新的基因、开发新的生物活性物质，了解未培微生物多样性，而且还能获得生物遗传、代谢和生理等方面信息以及在不同生境条件下动物胃肠道微生物区系变化规律、信号通路及响应机制。论文简述了宏基因组文库的构建和分析方法，重点介绍了宏基因组技术在动物胃肠道微生物应用中的研究进展。
동물위장도중존재복잡다양적미생물，단지유소수미생물능구용전통방법진행순배양。이용굉기인조학방법연구동물위장도미생물요과순배양기술，불부가이발현신적기인、개발신적생물활성물질，료해미배미생물다양성，이차환능획득생물유전、대사화생리등방면신식이급재불동생경조건하동물위장도미생물구계변화규률、신호통로급향응궤제。논문간술료굉기인조문고적구건화분석방법，중점개소료굉기인조기술재동물위장도미생물응용중적연구진전。
T here are enormous and complicated microbes in the animal gastrointestinal ecosystem ,but only few microorganism can be cultured by traditional method .Using metagenomics to study gastrointestinal tract of animals can not only find new genes ,explore new bioactivators and understand the diversity of un-cultured microorganism ,but also can require the informations of biological heredity ,metabolism and physi-ology and the change rule ,signal path and response mechanism in animal gastrointestinal ecosystem under the different habitat conditions .This paper reviewed some methodologies in metagenome library construc-tion and screening ,and especially introduced the application of metagenomics in animal gastrointestinal eco-system .