CAJ | 학술논문

为建立鉴定普氏野马的PCR方法，根据线粒体D-Loop区序列，设计了针对普氏野马的特异性扩增引物，通过优化反应体系和扩增条件，建立了能够鉴定普氏野马的PCR方法。该方法检测灵敏度较高，可扩增10-3 ng的DNA ，与蒙古马、伊犁马、哈萨克马、阿拉伯马、驴、骡、牛和羊等动物均不发生交叉反应，具有很好的特异性。结果表明，建立的普氏野马物种鉴定的PCR方法特异性好，灵敏度较高，简单可靠，可作为普氏野马的一种有效鉴定方法，对普氏野马的保护起到重要的作用。
위건립감정보씨야마적PCR방법，근거선립체D-Loop구서렬，설계료침대보씨야마적특이성확증인물，통과우화반응체계화확증조건，건립료능구감정보씨야마적PCR방법。해방법검측령민도교고，가확증10-3 ng적DNA ，여몽고마、이리마、합살극마、아랍백마、려、라、우화양등동물균불발생교차반응，구유흔호적특이성。결과표명，건립적보씨야마물충감정적PCR방법특이성호，령민도교고，간단가고，가작위보씨야마적일충유효감정방법，대보씨야마적보호기도중요적작용。
To develop the species-specific PCR assay for the identification of Equus przewalsrii ,primers were designed from mitochondria D-loop region after alignment of the available sequences in the GenBank database .The primers generated specific fragments of 315 bp length for Equus przewalsrii .The results showed that the PCR was established .The sensitivity analysis showed that the developed PCR could detect 10-3 ng DNA ,and no cross reaction with Mongolia horse ,Ili horse ,Kazakh horse ,Arabian horse ,Don-key ,Mule ,Bovine and Ovine .The results demonstrated that this species-specific PCR provided a useful , specific ,sensitive and simple technique to identify Equus przewalsrii .This assay could play an important role in conservation of Equus p rz ew alsrii .