CAJ | 학술논문

为鉴定潜在的松材线虫防治靶标基因，应用SL法克隆到松材线虫Rbx1，并完成Rbx1蛋白质结构分析。松材线虫Rbx1与泛素途径多个蛋白发生互作。通过Rbx1与E2的分子对接研究，共发现Rbx1与E2分子间存在3个氢键，Rbx1含有1个E2契合“口袋”。分子动力学模拟表明，Rbx1的氮末端是其功能区域。松材线虫Rbx1是潜在的防治靶标基因，以该区域为靶位点设计抑制子或可达到防治该线虫的目的。
위감정잠재적송재선충방치파표기인，응용SL법극륭도송재선충Rbx1，병완성Rbx1단백질결구분석。송재선충Rbx1여범소도경다개단백발생호작。통과Rbx1여E2적분자대접연구，공발현Rbx1여E2분자간존재3개경건，Rbx1함유1개E2계합“구대”。분자동역학모의표명，Rbx1적담말단시기공능구역。송재선충Rbx1시잠재적방치파표기인，이해구역위파위점설계억제자혹가체도방치해선충적목적。
An Rbx1 enzyme gene entire cDNA was cloned from Bursaphelenchus xylohp ilus.The structure and functions of the Rbx1 were analyzed .The Rbx1 and E2 interface was supported by several obvious hydrogen bonding interactions .From the docking of Rbx1 and E2, residues of E2 binding to the central groove provided by the Rbx1.By the molecular dynamics simulation, the N terminus of Rbx1 are important in the binding.The N terminus can be used as the repressor binding site to control the B.xylophilus.Therefore, Rbx1 is a potential target for developing potent antinematodal drugs against B.xy-lophilus.