东北林业大学学报
東北林業大學學報
동북임업대학학보
JOURNAL OF NORTHEAST FORESTRY UNIVERSITY
2013年
11期
59-62
,共4页
OT杂种系百合%染色体%核型分析
OT雜種繫百閤%染色體%覈型分析
OT잡충계백합%염색체%핵형분석
OT hybrid lily%Chromosome%Karyotype analysis
采用常规根尖压片法，对百合OT杂种系的‘Nymph ’、‘Valparaiso ’和‘Yelloween ’3个品种进行了核型分析。因OT系列的品种为东方百合和喇叭百合杂种系间的杂交品种，染色体高度杂合，核型复杂，故将每条染色体分别列出单独分析。研究结果显示：‘Valparaiso’为三倍体，其余两种为二倍体。3品种均为3B型，核型不对称系数分别为79．79％、80．15％和81．73％。其中‘Valparaiso’可能存在B染色体。核型公式分别为‘Nymph’2n＝2x＝24＝2m（SAT）＋2sm＋9t＋11st；‘Valparaiso’3n＝3x＝36＝4m（SAT）＋3sm（SAT）＋15st＋14t；‘Yelloween’2n＝2x＝24＝4m（2SAT）＋16t＋4st。
採用常規根尖壓片法，對百閤OT雜種繫的‘Nymph ’、‘Valparaiso ’和‘Yelloween ’3箇品種進行瞭覈型分析。因OT繫列的品種為東方百閤和喇叭百閤雜種繫間的雜交品種，染色體高度雜閤，覈型複雜，故將每條染色體分彆列齣單獨分析。研究結果顯示：‘Valparaiso’為三倍體，其餘兩種為二倍體。3品種均為3B型，覈型不對稱繫數分彆為79．79％、80．15％和81．73％。其中‘Valparaiso’可能存在B染色體。覈型公式分彆為‘Nymph’2n＝2x＝24＝2m（SAT）＋2sm＋9t＋11st；‘Valparaiso’3n＝3x＝36＝4m（SAT）＋3sm（SAT）＋15st＋14t；‘Yelloween’2n＝2x＝24＝4m（2SAT）＋16t＋4st。
채용상규근첨압편법，대백합OT잡충계적‘Nymph ’、‘Valparaiso ’화‘Yelloween ’3개품충진행료핵형분석。인OT계렬적품충위동방백합화나팔백합잡충계간적잡교품충，염색체고도잡합，핵형복잡，고장매조염색체분별렬출단독분석。연구결과현시：‘Valparaiso’위삼배체，기여량충위이배체。3품충균위3B형，핵형불대칭계수분별위79．79％、80．15％화81．73％。기중‘Valparaiso’가능존재B염색체。핵형공식분별위‘Nymph’2n＝2x＝24＝2m（SAT）＋2sm＋9t＋11st；‘Valparaiso’3n＝3x＝36＝4m（SAT）＋3sm（SAT）＋15st＋14t；‘Yelloween’2n＝2x＝24＝4m（2SAT）＋16t＋4st。
The karyotype of three representative new cultivars from OT Hybrid Lilies, ‘Nymph’, ‘Valparaiso’ and ‘Yellow-een’ were taken as samples for analysis by traditional squash method.The chromosomes were listed for analysis separately considering the karyotypic complexity of the OT lily as a intersectional hybrid.‘Valparaiso’ is triploid, and the other two cultivars are diploid.Karyotypes of all the Lilium cultivars are 3B, and the asymmetry index ( As· K%) are 79.79%, 80.18%and 81.73%, respectively.B chromosomes may be in‘Valparaiso’.The karyotype formula of‘Nymph’ is 2n=2x=24=2m(SAT)+2sm+9t+11st, that of ‘Valparaiso’ is 3n=3x=36=4m(SAT)+3sm(SAT)+15st+14t, and that of‘Yelloween’ is 2n=2x=24=4m(2SAT)+16t+4st.
