CAJ | 학술논문

目的：采用反相高效液相色谱法建立同时测定马钱子药材及其总碱提取物中马钱子碱和士的宁含量的方法。方法采用Licrospher C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈∶0.01 mol/mL庚烷磺酸钠与0.02 mol/mL磷酸二氢钾等体积混合溶液(用10%磷酸调pH值为2.8)＝27∶73,检测波长260 nm,柱温30℃,流速为1 mL/min。结果马钱子碱在0.1～1.0μg、士的宁在0.12～1.2μg 范围内线性关系良好(r＝1.000),平均回收率分别为99.88%(RSD＝1.06%)、100.06%(RSD＝0.78%)。结论本方法操作可靠、精确,可用于中药马钱子药材及提取物的含量测定。
목적：채용반상고효액상색보법건립동시측정마전자약재급기총감제취물중마전자감화사적저함량적방법。방법채용Licrospher C18색보주(4.6 mm×250 mm,5μm),류동상위을정∶0.01 mol/mL경완광산납여0.02 mol/mL린산이경갑등체적혼합용액(용10%린산조pH치위2.8)＝27∶73,검측파장260 nm,주온30℃,류속위1 mL/min。결과마전자감재0.1～1.0μg、사적저재0.12～1.2μg 범위내선성관계량호(r＝1.000),평균회수솔분별위99.88%(RSD＝1.06%)、100.06%(RSD＝0.78%)。결론본방법조작가고、정학,가용우중약마전자약재급제취물적함량측정。
Objective To develop a RP-HPLC method for the determination of Brucine and Strychnine in Semen Strychni and its extractive of total alkaloids. Methods A chromatographic column of Licrospher C18 (4.6 mm×250 mm, 5 μm) was used with the mobile phase of acetonitrile∶0.01 mol/L sodium heptane sulfonate and 0.02 mol/L potassium dihydrogen phosphate mixed with equal amount (adjusted pH to 2.8 with 10% phosphonic acid)=27∶73, detection wavelength at 260 nm, column temperature of 30 ℃ and flow rate of 1 mL/min. Results The calibration curves of Brucine and Strychnine were both in good linearity in the ranges of 0.1-1.0 μg and 0.12-1.2 μg (r=1.000) respectively. The average recovery rates of Brucine and Strychnine were 99.88% (RSD=1.06%) and 100.06% (RSD=0.78%) respectively. Conclusion The method is realiable and accurate, which can be applied to determination of Brucine and Strychnine in Semen Strychi and its extractive.