CAJ | 학술논문

目的：建立高效液相色谱法(HPLC)测定含油脂类药材中低分子羰基化合物的方法。方法苦杏仁等药材经水提取,在酸性条件下,经2,4-二硝基苯肼(DNPH)衍生后,衍生物直接用HPLC测定。色谱柱：Kromasil KR100-5 C18(250 mm×4.6 mm,5μm)；流动相：A为水-乙腈-四氢呋南-异丙醇(59∶30∶10∶1),B为水-乙腈(35∶65),梯度洗脱；流速：0.8 mL/min；检测波长：365 nm；柱温：30℃。结果甲醛、乙醛、丙酮、丙烯醛、丙醛、巴豆醛、2-丁酮、丁醛的DNPH衍生物在一定浓度范围内与峰面积线性关系良好,相关系数均达到0.999以上,平均回收率为88.49%～93.65%,最低检测限为0.002～0.008μg/mL。结论该方法简便、灵敏、准确、重复性较好。
목적：건립고효액상색보법(HPLC)측정함유지류약재중저분자탄기화합물적방법。방법고행인등약재경수제취,재산성조건하,경2,4-이초기분정(DNPH)연생후,연생물직접용HPLC측정。색보주：Kromasil KR100-5 C18(250 mm×4.6 mm,5μm)；류동상：A위수-을정-사경부남-이병순(59∶30∶10∶1),B위수-을정(35∶65),제도세탈；류속：0.8 mL/min；검측파장：365 nm；주온：30℃。결과갑철、을철、병동、병희철、병철、파두철、2-정동、정철적DNPH연생물재일정농도범위내여봉면적선성관계량호,상관계수균체도0.999이상,평균회수솔위88.49%～93.65%,최저검측한위0.002～0.008μg/mL。결론해방법간편、령민、준학、중복성교호。
Objective To establish an HPLC method for determination of low-molecular-weight carbonyl compounds in the oil-containing herbs. Methods After carbonyl compounds in the samples were extracted with water, the solution reacted with 2, 4-dinitrophenylhydrazine (DNPH) in an acidic medium to form 2, 4-dinitrophenylhydrazone derivatives, which were separated on Kromasil KR100-5 C18 column (250 mm× 4.6 mm, 5 μm). The mobile phase A was water-acetonitrile-tetrahydrofuran-isopropanol (59∶30∶10∶1), mobile phase B was water-acetonitrile (35∶65), gradient elution. The flow rate was 0.8 mL/min, detection wavelength was 365 nm, and column temperature was 30 ℃. Results Good linearities were obtained in corresponding concentration ranges, with correlation coefficient over 0.999. The limits of detection of the eight DNPH derivatives were 0.002-0.008 μg/mL, and the average recoveries were 88.49%-93.65%. Conclusion The method is simple, accurate and reliable, with good reproducibility.