CAJ | 학술논문

目的：观察四妙勇安汤合生脉饮对缺氧/复氧乳鼠心肌细胞细胞凋亡的影响，探讨其心肌细胞保护作用。方法采用胰蛋白和胶原酶联合消化的方法原代培养的 SD 乳鼠心肌细胞，利用高纯氮气建立心肌细胞缺氧/复氧模型。实验分为正常组、模型组和药物干预组(予四妙勇安汤合生脉饮)。采用流式细胞术Annexin V-FITC/PI双染法检测心肌细胞凋亡率。结果与正常组比较，模型组及药物干预组心肌细胞凋亡率均升高(P＜0.05)。与模型组比较，药物干预组心肌细胞凋亡率下降(P＜0.05)。结论四妙勇安汤合生脉饮对缺氧/复氧后心肌细胞凋亡有保护作用。
목적：관찰사묘용안탕합생맥음대결양/복양유서심기세포세포조망적영향，탐토기심기세포보호작용。방법채용이단백화효원매연합소화적방법원대배양적 SD 유서심기세포，이용고순담기건립심기세포결양/복양모형。실험분위정상조、모형조화약물간예조(여사묘용안탕합생맥음)。채용류식세포술Annexin V-FITC/PI쌍염법검측심기세포조망솔。결과여정상조비교，모형조급약물간예조심기세포조망솔균승고(P＜0.05)。여모형조비교，약물간예조심기세포조망솔하강(P＜0.05)。결론사묘용안탕합생맥음대결양/복양후심기세포조망유보호작용。
Objective To observe the effect of Simiaoyongan decoction with Shengmai decoction on apoptosis of hypoxia/reoxygenation model of primary cultured neonatal rat cardiomyocytes. Methods The combination of trypsin and collagenase was used to isolate neonatal rat myocardial cells. Hypoxia/reoxygenation model of cardiomyocytes was made by high purity nitrogen gas. Primary cultured neonatal rat cardiomyocytes were randomly divided into control group, model group and treatment group (treated with Simiaoyongan decoction and Shengmai decoction). The myocardial apoptotic rates were detected by Annexin V-FITC/PI double-staining method. Results Compared with the control group, the apoptotic rate of model group and treatment group was high (P<0.05). Drug intervention lowered the apoptotic rate compared with the model group, the difference was significant (P <0.05). Conclusion The alcohol sedimentation solution of Simiaoyongan decoction with Shengmai decoction played a protective role on hypoxia/reoxygenation cardiomyocytes.