CAJ | 학술논문

目的：观察香青兰总黄酮对支气管哮喘大鼠肺功能及肺组织Ⅲ型胶原的影响。方法 SD 大鼠60只随机分为正常对照组、模型组、地塞米松组和香青兰总黄酮低、中、高剂量组。除正常对照组外,其余各组采用卵白蛋白(OVA)致敏和激发建立大鼠哮喘模型。模型建立后,正常对照组和模型组给予双蒸水灌胃,各给药组分别给予香青兰总黄酮和地塞米松灌胃,1次/d,连续30 d。末次给药后,采用美国Buxco公司的整体体积描记系统,检测香青兰总黄酮对支气管哮喘大鼠呼吸频率(F)、每分钟通气量(MVb)、吸气流量峰值(PIFb)及呼气流量峰值(PEFb)及增强的呼吸间歇(Penh)的影响,ELISA 法检测肺组织Ⅲ型胶原含量变化。结果香青兰总黄酮180、360 mg/kg 可降低支气管哮喘大鼠 F 和 Penh(P＜0.05,P＜0.01),升高 MVb、PIFb 和 PEFb(P＜0.05),降低肺组织Ⅲ型胶原含量(P＜0.05,P＜0.01)。结论香青兰总黄酮可改善支气管哮喘大鼠肺功能,降低气道反应性,改善哮喘气道重塑。
목적：관찰향청란총황동대지기관효천대서폐공능급폐조직Ⅲ형효원적영향。방법 SD 대서60지수궤분위정상대조조、모형조、지새미송조화향청란총황동저、중、고제량조。제정상대조조외,기여각조채용란백단백(OVA)치민화격발건립대서효천모형。모형건립후,정상대조조화모형조급여쌍증수관위,각급약조분별급여향청란총황동화지새미송관위,1차/d,련속30 d。말차급약후,채용미국Buxco공사적정체체적묘기계통,검측향청란총황동대지기관효천대서호흡빈솔(F)、매분종통기량(MVb)、흡기류량봉치(PIFb)급호기류량봉치(PEFb)급증강적호흡간헐(Penh)적영향,ELISA 법검측폐조직Ⅲ형효원함량변화。결과향청란총황동180、360 mg/kg 가강저지기관효천대서 F 화 Penh(P＜0.05,P＜0.01),승고 MVb、PIFb 화 PEFb(P＜0.05),강저폐조직Ⅲ형효원함량(P＜0.05,P＜0.01)。결론향청란총황동가개선지기관효천대서폐공능,강저기도반응성,개선효천기도중소。
Objective To investigate the effect of moldavica total flavone on pulmonary function and collagen type Ⅲ in lung tissue of ovalbumin-induced bronchial asthmatic rats. Methods A total of 60 SD rats were randomly divided into control group, model group, dexamethasone group, and moldavica total flavone low, medium and high dose group. Rat asthma model was established by ovalbumin challenge methods except the control group. The control and model group were intragastrically given distilled water, medicine groups were intragastrically given moldavica total flavone and dexamethasone for 30 d, once per day. After the last administration, the pulmonary function and airway responsiveness including breathing frequency (F), minute ventilation (MVb), peak inspiratory flow (PIFb), peak expiratory flow (PEFb) and enhanced pause (Penh) were measured by using non-invasive measurement system (Penh system, Buxco, USA). The collagen type Ⅲ in lung tissue were detected by ELISA. Results The moldavica total flavone at 180 and 360 mg/kg decreased F and Penh (P<0.05, P<0.01), increased MVb, PIFb and PEFb (P<0.05), and decreased collagen type Ⅲ in lung tissue (P<0.05, P<0.01). Conclusion Moldavica total flavone can relieve the airway hyperresponsiveness and remodeling, improve respiratory function of ovalbumin-induced bronchial asthmatic rats.