CAJ | 학술논문

目的：探讨甲状腺乳头状癌（PTC）中特征性的miRNA表达谱以及临床病理意义。方法对52例PTC及7例良性甲状腺肿瘤手术切除标本采用基因芯片技术及RNA印迹杂交法检测miRNA的表达，对过表达的miRNA表达水平与PTC临床病理特征之间的关系进行分析。结果（1）用基因芯片技术筛检发现 PTC 组织中5例过表达的 miRNAs（miR-375、miR-34a、miR-146b、miR-222、miR-31）。（2）RNA印迹杂交法验证芯片结果及检测其他miRNAs结果显示：miR-34a、miR-146b、miR-31、miR-21和miR-221在PTC组织中的表达水平较癌旁正常甲状腺组织显著升高（P＜0.05）。MiR-146b和miR-221在PTC组织中的过表达率较良性甲状腺肿瘤组织显著升高（P＜0.01）。PTC患者中包膜侵犯组、淋巴结转移组、TNM分期III- IV期组及AGES系统评分≥5分组miR-221的表达水平均明显高于相应对照组（P＜0.05或0.01）；而男性PTC患者的miR-146b表达水平明显高于女性PTC患者（P＜0.05）。结论 miR-146b、miR-221、miR-21和miR-31的过表达与PTC的发生、发展有关；过表达的miR-221和miR-146b与PTC的预后不良有关。
목적：탐토갑상선유두상암（PTC）중특정성적miRNA표체보이급림상병리의의。방법대52례PTC급7례량성갑상선종류수술절제표본채용기인심편기술급RNA인적잡교법검측miRNA적표체，대과표체적miRNA표체수평여PTC림상병리특정지간적관계진행분석。결과（1）용기인심편기술사검발현 PTC 조직중5례과표체적 miRNAs（miR-375、miR-34a、miR-146b、miR-222、miR-31）。（2）RNA인적잡교법험증심편결과급검측기타miRNAs결과현시：miR-34a、miR-146b、miR-31、miR-21화miR-221재PTC조직중적표체수평교암방정상갑상선조직현저승고（P＜0.05）。MiR-146b화miR-221재PTC조직중적과표체솔교량성갑상선종류조직현저승고（P＜0.01）。PTC환자중포막침범조、림파결전이조、TNM분기III- IV기조급AGES계통평분≥5분조miR-221적표체수평균명현고우상응대조조（P＜0.05혹0.01）；이남성PTC환자적miR-146b표체수평명현고우녀성PTC환자（P＜0.05）。결론 miR-146b、miR-221、miR-21화miR-31적과표체여PTC적발생、발전유관；과표체적miR-221화miR-146b여PTC적예후불량유관。
Objective To investigate expression of specific miRNAs in papillary thyroid carcinoma (PTC) and their clini-copathological significance. Methods Fifty two surgical specimens of PTC tissues and 7 specimens of benign thyroid tumor tis-sues were col ected. The miRNA expression levels were detected by using microarray and Northern blot. The correlation between over- expressed miRNAs and clinicopathological features of PTC were analyzed. Results A set of 5 over- expressed miRNAs (miR- 375, miR- 34a, miR- 146b, miR- 222, miR- 31) was distinguished by microarray in PTC tissues compared to cancer- adja-cent tissues. Using Northern blot, the results of microarray were verified;and Northern blot results also showed that the expres-sion levels of miR- 34a, miR- 146b, miR- 31, miR- 221 and miR- 21 in PTC were significantly higher than those in cancer- adjacent tissues(P<0.05). Additional y, the over- expression rates of miR- 146b and miR- 221 in PTC tissues were significantly higher than those in benign thyroid tumor tissues (P<0.01). The expression levels of miR- 221 in PTC patients with extrathyroidal invasion, lymph node metastasis, advanced TNM stage (III- IV) and AGES score≥5 were significantly higher than those in control groups (P<0.05 or 0.01);whereas the expression level of miR- 146b in male PTC patients was significantly higher than that in female PTC patients (P<0.05). Conclusion Over- expression of miR- 146b, miR- 221, miR- 21, and miR- 31 may be associated with car-cinogenesis and progression of PTC, and the over- expression of miR- 221 and miR- 146b are also correlated with poor clinical outcome of PTC patients.