CAJ | 학술논문

目的：观察心肌细胞核转录因子-κB（ NF-κB）在脓毒症大鼠心肌细胞的表达，并探讨辛伐他汀预处理对其表达的影响及意义。方法雌性Wistar大鼠60只随机分为3组：正常组，脓毒症组和辛伐他汀组，每组20例。处理方法：应用盲肠结扎穿孔法制造脓毒症模型。辛伐他汀组：辛伐他汀20 mg/kg喂养，每天1次，共2周。2周后辛伐他汀组与脓毒症组大鼠行盲肠结扎穿孔术。术后48 h取3组大鼠心肌组织制成石蜡切片，采用免疫组织化学染色的方法观察心肌细胞NF-κB的蛋白表达。结果免疫组织化学染色显示脓毒症组大鼠心肌细胞NF-κB蛋白表达较正常组明显增加（ P ＜0抖．05），辛伐他汀组大鼠心肌细胞NF-κB 蛋白表达与脓毒症组相比明显减弱（ P ＜0．05），与正常组相比差异有统计学意义（ P ＜0．05）。结论辛伐他汀可以减弱脓毒症大鼠心肌细胞NF-κB的蛋白表达，改善脓毒症时的心脏功能。
목적：관찰심기세포핵전록인자-κB（ NF-κB）재농독증대서심기세포적표체，병탐토신벌타정예처리대기표체적영향급의의。방법자성Wistar대서60지수궤분위3조：정상조，농독증조화신벌타정조，매조20례。처리방법：응용맹장결찰천공법제조농독증모형。신벌타정조：신벌타정20 mg/kg위양，매천1차，공2주。2주후신벌타정조여농독증조대서행맹장결찰천공술。술후48 h취3조대서심기조직제성석사절편，채용면역조직화학염색적방법관찰심기세포NF-κB적단백표체。결과면역조직화학염색현시농독증조대서심기세포NF-κB단백표체교정상조명현증가（ P ＜0두．05），신벌타정조대서심기세포NF-κB 단백표체여농독증조상비명현감약（ P ＜0．05），여정상조상비차이유통계학의의（ P ＜0．05）。결론신벌타정가이감약농독증대서심기세포NF-κB적단백표체，개선농독증시적심장공능。
Objce tive To investigate the expression of NF-κB in cardiac myocytes of rats with sepsis and to explore the effects of simvastatin pretreatment on expression of NF-κB and its significance .Methods Sixty female Wistar rats were randomly divided into three groups:control group ,sepsis group and simvastatin group ,with 20 rats in each group.The animal models with sepsis were established by cecal ligation and puncture (CLP).The rats in simvastatin group were treated with simvastatin ,20mg/kg,once a day for two weeks before CLP .After two weeks the rats in simvastatin group and sepsis group received CLP .The cardiac tissues of the three groups were collected 48h after CLP, and all the specimens were made into paraffin sections , then the expression levels of NF-κB were detected by immunohistochemistry staining .Resutl s Immunohistochemistry staining showed that the expression levels of NF-κB in sepsis group were significantly higher than those in control group 48h after CLP (P <0.05).The expression levels of NF-κB in simvastatin group were significantly lower than those in sepsis group (P <0.05),furthermore,there were significant differences between control group and simvastatin group (P <0.05).Conlc usion Simvastatin can decrease the expression of NF-κB in cardiac myocytes of rats with sepsis,as a result,which can improve heart function .