山西医药杂志
山西醫藥雜誌
산서의약잡지
SHANXI MEDICAL JOURNAL
2013年
22期
1214-1216
,共3页
乳腺疾病%克隆 ,分子%聚合酶链反应
乳腺疾病%剋隆 ,分子%聚閤酶鏈反應
유선질병%극륭 ,분자%취합매련반응
Breast diseases%Cloning,molecular%Polymerase chain reaction
目的：探讨各级导管内增生性病变的克隆组成，以判断病变是否为肿瘤性增生。方法选取80例女性导管内增生性病变，包括普通导管增生（UDH）20例，平坦型上皮非典型增生（FEA）15例，非典型导管增生（ADH）20例，导管内原位癌（DCIS）25例，另外选取20份正常乳腺组织作为对照，应用显微切割技术分离所需病变，提取基因组DNA ，经甲基化敏感的 Hha Ⅰ消化，巢式聚合酶链反应（PCR）扩增雄激素受体（AR）基因，应用变性聚丙烯酰胺凝胶电泳显示AR基因的CAG重复序列长度多态性。结果100例标本中91例扩增成功；所有的DCIS均为单克隆性增生，所有正常组织和大多数的UDH （94％，17/18）为多克隆性增生，27％（3/11）的FEA及71％（12/17）的ADH为单克隆性增生。结论大部分的ADH及部分FEA ，所有的DCIS为单克隆性增生，为肿瘤性增生。
目的：探討各級導管內增生性病變的剋隆組成，以判斷病變是否為腫瘤性增生。方法選取80例女性導管內增生性病變，包括普通導管增生（UDH）20例，平坦型上皮非典型增生（FEA）15例，非典型導管增生（ADH）20例，導管內原位癌（DCIS）25例，另外選取20份正常乳腺組織作為對照，應用顯微切割技術分離所需病變，提取基因組DNA ，經甲基化敏感的 Hha Ⅰ消化，巢式聚閤酶鏈反應（PCR）擴增雄激素受體（AR）基因，應用變性聚丙烯酰胺凝膠電泳顯示AR基因的CAG重複序列長度多態性。結果100例標本中91例擴增成功；所有的DCIS均為單剋隆性增生，所有正常組織和大多數的UDH （94％，17/18）為多剋隆性增生，27％（3/11）的FEA及71％（12/17）的ADH為單剋隆性增生。結論大部分的ADH及部分FEA ，所有的DCIS為單剋隆性增生，為腫瘤性增生。
목적：탐토각급도관내증생성병변적극륭조성，이판단병변시부위종류성증생。방법선취80례녀성도관내증생성병변，포괄보통도관증생（UDH）20례，평탄형상피비전형증생（FEA）15례，비전형도관증생（ADH）20례，도관내원위암（DCIS）25례，령외선취20빈정상유선조직작위대조，응용현미절할기술분리소수병변，제취기인조DNA ，경갑기화민감적 Hha Ⅰ소화，소식취합매련반응（PCR）확증웅격소수체（AR）기인，응용변성취병희선알응효전영현시AR기인적CAG중복서렬장도다태성。결과100례표본중91례확증성공；소유적DCIS균위단극륭성증생，소유정상조직화대다수적UDH （94％，17/18）위다극륭성증생，27％（3/11）적FEA급71％（12/17）적ADH위단극륭성증생。결론대부분적ADH급부분FEA ，소유적DCIS위단극륭성증생，위종류성증생。
Objective To demonstrate the clonality status of intraductal proliferative lesions .Methods Four groups′samples ,including 20 cases of usual ductal hyperplasia (UDH) ,15 cases of flat epithelia atypia (FEA) , 20 cases of atypical ductal hyperplasia (ADH) ,and 25 cases of ductal carcinoma in situ (DCIS) were selected for analysis .Twenty specimens of normal breast tissue were used as a control group .Microdissection was performed to collect the tissue samples for extraction of genomic DNA from paraffin-embedded tissues .The DNA was subjec-ted to nested-PCR amplification of the CAG repeats in androgen receptor (AR) gene exon Ⅰ with and without pri-or digestion of methylation-sensitive restriction enzyme Hha Ⅰ .Gel electrophoresis was used to detect the clonal nature of these four groups′samples .Results DNA from 91 of 101 patients was able to be amplified at the AR gene exon Ⅰ .The analysis confirmed monoclonality in all informative samples of DCIS cells .Normal tissues and the majority (94% ) of UDH were shown to be polyclonal .Monoclonality was revealed in 12/17 (71% ) cases of ADH .Among 11 cases of FEA ,8 cases were shown to be polyclonal ,while 3 cases displayed monoclonal altera-tions which accounted for 27% .Conclusion Most of ADH ,the smaller part of FEA and all DCIS have clonal al-terations ,which may be neoplastic lesions .
