中外医学研究
中外醫學研究
중외의학연구
CHINESE AND FOREIGN MEDICAL RESEARCH
2014年
14期
137-139
,共3页
何艳%张锦泉%邓阳勇%张水波
何豔%張錦泉%鄧暘勇%張水波
하염%장금천%산양용%장수파
分子信标%荧光共振能量转移%腺苷
分子信標%熒光共振能量轉移%腺苷
분자신표%형광공진능량전이%선감
Molecular beacon%Fluorescence resonance energy transfer%Adenosine
目的：利用荧光共振能量转移原理和适体分子信标探针技术开发腺苷检测体系。方法：以金纳米粒子作为荧光共振能量转移受体。结果：在最适条件下，腺苷浓度在2.0×10-8~1.8×10-6 mol/L范围内线性关系良好(r=0.9961)，腺苷的检出限为6.0×10-9 mol/L，腺苷测定的回收率分别为97.6%、102.1%、105.8%。结论：将新方法应用于实际尿样中腺苷的检测，与高效液相色谱法比较差异无统计学意义。
目的：利用熒光共振能量轉移原理和適體分子信標探針技術開髮腺苷檢測體繫。方法：以金納米粒子作為熒光共振能量轉移受體。結果：在最適條件下，腺苷濃度在2.0×10-8~1.8×10-6 mol/L範圍內線性關繫良好(r=0.9961)，腺苷的檢齣限為6.0×10-9 mol/L，腺苷測定的迴收率分彆為97.6%、102.1%、105.8%。結論：將新方法應用于實際尿樣中腺苷的檢測，與高效液相色譜法比較差異無統計學意義。
목적：이용형광공진능량전이원리화괄체분자신표탐침기술개발선감검측체계。방법：이금납미입자작위형광공진능량전이수체。결과：재최괄조건하，선감농도재2.0×10-8~1.8×10-6 mol/L범위내선성관계량호(r=0.9961)，선감적검출한위6.0×10-9 mol/L，선감측정적회수솔분별위97.6%、102.1%、105.8%。결론：장신방법응용우실제뇨양중선감적검측，여고효액상색보법비교차이무통계학의의。
Objective:A new method for the detection of adenosine has been established by using fluorescence resonance energy transfer and aptamer molecular beacon.Method:Using gold nanoparticles as fluorescence resonance energy transfer recepter.Result:Under the selected condition ,the concentration of adenosine in the range of 2.0×10-8-1.8×10-6 mol/L(r=0.9961).The limit of detection for adenosine was 6.0×10-9 mol/L.The recoveries were 97.6%,102.1%, 105.8%.Conclusion:The present method has been applied to determination of adenosine in real urine samples,and the obtained results were in good agreement with those obtained by the HPLC method.
